扬州大学学报（农业与生命科学版）2011，Vol.32Issue(1)：55-58,4.
非洲猪瘟病毒p72基因的纳米金探针研究
Study on the nanogold probe to p72 gene of African swine fever virus
摘要
Abstract
After comparing the p72 genes of 22 African swine fever virus strains, a highly conserved sequence was found, to which two complementary oligonucleotide probes modified with 5＇-biotin and 3＇-alkylthiol respectively were designed and synthesized. The alkylthiol oligonucleotides were attached to gold nanoparticles by covalent coupling, and the gold nanoparticle probe was prepared. The conservative DNA about 651 bp in p72 gene amplified by PCR was denatured,1which then was hybridized with the probes above. The hybrid products were added into the ELISA plate labeled with streptavidin and captured by affinity. After that, the siliver staining was proceeded to amplify the hybrid signal. The result indicated that the black deposition can be viewed after siliver enhancement,and the DNA amplified can be detected with the lowest the density at 10 fmol · L-1 , which may be used in rapid detection of the African swine fever virus in future.关键词
纳米金探针/非洲猪瘟病毒/p72基因/检测/银染增强Key words
Nanogold probe/ ASFV/ p72 gene/ detection/ siliver enhancement分类
农业科技引用本文复制引用
张鑫宇,孙怀昌,刘文俊,夏晓莉,成大荣..非洲猪瘟病毒p72基因的纳米金探针研究[J].扬州大学学报（农业与生命科学版）,2011,32(1):55-58,4.基金项目
国家高技术研究发展计划项目(863-2007AA10Z419) （863-2007AA10Z419）
