中国病理生理杂志2011，Vol.27Issue(6)：1132-1137,6.DOI:10.3969/j.issn.1000-4718.2011.06.016
缺氧后处理中CYP2J3/EETs系统对心肌凋亡的影响
Effects of CYP2J3/EETs on myocardial apoptosis under the condition of hypoxic postconditioning
摘要
Abstract
AIM: To investigate the effects and underlying mechanism of endogenous cytochrome P450 2J3/epoxyeicosatrienoic acids ( CYP2J3/EETs ) system on myocardial apoptosis under the condition of hypoxic postconditioning ( HPost ).METHODS: Primary myocardial cells from neonatal Wistar rats ( 12 h - 24 h ) were cultured and divided into 7 groups as follows: control group, hypoxia/reoxygenation ( H/R ) group, HPost group, CYP2J3 transfection group, empty vector group, 6 - ( 2 - propargyloxyphenyl )hexanoic acid ( PPOH, an inhibitor of CYP2J3 ) group and dimethyl sulfoxide ( DMSO, as solvent) group.The H/R treatment was conducted in all the groups except control group.The cell viability was tested by MTT method.The concentration of 11,12 - EET in the cell culture medium was measured by high - performance liquid chromatography ( HPLC ).The expression of caspase - 3 was detected by Western blotting and its activity was determined by caspase -3 activity assay kit.RESULTS: Compared with H/R group, the cell viability and the 11,12 -EET concentration were significantly elevated in HPost group ( P ＜0.01 ).The two indexes in CYP2J3 transfection group were higher than those in HPost group ( P ＜0.01 ), but they were lowered in PPOH group than those in HPost group ( P ＜ 0.01 ).The comparisons of the expression and activity of caspase -3 among groups were as follows: H/R group ＞ HPost group ( P ＜0.01 ), PPOH group ＞ HPost group ( P ＜0.01 ) and empty vector group ＞ CYP2J3 transfection group ( P ＜ 0.05 ).Additionally, the caspase - 3 expression in CYP2J3 transfection group was lower than that in HPost group ( P ＜ 0.01 ).CONCLUSION: The CYP2J3/EETs system protects myocardial cells through inhibiting caspase -3 - induced apoptosis.关键词
缺氧后处理/缺氧/复氧损伤/心肌细胞/细胞色素P450表氧化酶2J3/环氧二十碳三烯酸/半胱氨酸天冬氨酸蛋白酶/3Key words
Hypoxic postconditioning/ Hypoxia/reoxygenation injury/ Cardiomyocytes/ Cytochrome P450 2J3 / Epoxyeicosatrienoic acids/ Caspase - 3分类
医药卫生引用本文复制引用
赵艳芝,张冬梅,于刚刚,曾翔俊,王红霞,芦玲巧,张立克,王岩梅..缺氧后处理中CYP2J3/EETs系统对心肌凋亡的影响[J].中国病理生理杂志,2011,27(6):1132-1137,6.基金项目
国家自然科学基金资助项目(No.30770874) （No.30770874）
