中国病理生理杂志2011，Vol.27Issue(6)：1143-1148,6.DOI:10.3969/j.issn.1000-4718.2011.06.018
p-ERK1/2-AP-1通路在姜黄素抗大鼠糖尿病神经病理性痛中的作用
Effect of curcumin on p-ERK1/2-AP-1 cascade and diabetic neuropathic pain in rats
摘要
Abstract
AIM: To evaluate the role of p - ERK1/2 - AP - 1 cascade in the process of curcumin against diabetic neuropathic pain ( DNP ) in rats.METHODS: Ninety - six male Sprague - Dawley rats were randomly divided into 4 groups ( n = 24 ): normal control group, DNP group, DNP with solvent group and DNP with curcumin ( 100 mg/kg ) group.The rat model of diabetes was induced by a single intraperitoneal injection of streptozotocin ( STZ, 75 mg/kg ).Mechanical allodynia and thermal hyperalgesia were tested by mechanical withdrawal threshold ( MWT ) and thermal withdrawal latency ( TWL ) 2 weeks after induction, respectively.The diabetic rats were treated with curcumin ( 100 mg·kg-1·d-1 , ip ) for 2 weeks.The conditions of hyperalgesia and allodynia were determined 2 d before STZ injection, 14 d after STZ injection, and 3 d, 7 d, 14 d after administered with curcumin.The change of p - ERK1/2 was measured by the methods of Western blotting and immunohistochemistry.The expression of AP - 1 in spinal cord dorsal horn and dorsal root ganglion ( DRG ) was detected by electromobility shift assay ( EMSA ).RESULTS: Compared with normal control group, the rats in DNP group developed hyperglycemia and a decrease in MWT and TWL associated with an increase in the activity of p - ERK1/ 2 and AP - 1 in dorsal horn and DRG( P ＜0.05 ).Compared with DNP group, 7 - day treatment with curcumin significantly attenuated mechanical allodynia and thermal hyperalgesia, and these effects were correlated with inhibiting the hyper -activation of p - ERK1/2 and AP - 1 14 days after treatment with curcumin ( P ＜0.05 ).CONCLUSION: Curcumin has beneficial effects on hyperalgesia in STZ - induced peripheral neuropathic pain.Activation of p - ERK1/2 and AP - 1 may be the key mechanism of DNP in spinal cord and DRG.关键词
姜黄素/糖尿病/神经病理性痛/p-ERK1/2-AP-1通路Key words
Curcumin/ Diabetes mellitus/ Neuropathic pain/ p - ERK1/2 - AP - 1 pathway分类
医药卫生引用本文复制引用
黄葱葱,陈果,吴艳,连庆泉,李军,曹红..p-ERK1/2-AP-1通路在姜黄素抗大鼠糖尿病神经病理性痛中的作用[J].中国病理生理杂志,2011,27(6):1143-1148,6.基金项目
国家自然科学基金资助项目(No.81073125) （No.81073125）
浙江省自然科学基金资助项目(No.Y2090252) （No.Y2090252）
浙江省科技厅基金资助项目(No.2006C14027) （No.2006C14027）
温州市科技局资助项目(No.H20070035) （No.H20070035）
