中国病理生理杂志2011，Vol.27Issue(6)：1176-1179,4.DOI:10.3969/j.issn.1000-4718.2011.06.024
巨噬细胞移动抑制因子经Rho途径促进人肺成纤维细胞III型胶原合成
Recombinant MIF induces synthesis of collagen type III in lung fibroblasts via Rho-kinase
摘要
Abstract
AIM: To investigate the effects of recombinant macrophage migration inhibitory factor ( rMIF ) on the synthesis of collagen type Ⅲ in fibroblasts.METHODS: The MRC - 5 fibroblasts were divided into 2 groups.The cells in treatment group were exposed to rMIF at the concentrations of 25 - 100 μg/L for 48 h.The cells without rMIF treatment served as control group.Half an hour before challenging with rMIF ( 100 μg/L ), Y27632 ( a Rho - kinase inhibitor ) was added to the cells in both 2 groups.After challenged for 48 h, total RNA and protein in the cells were extracted.The expression of collagen type Ⅲ at mRNA and protein levels was analyzed by RT - PCR and Western blotting, respectively.RESULTS : Different concentrations of rMIF significantly increased the expression of collagen type Ⅲ at mRNA and protein levels in a dose - dependent manner as compared with the control cells ( r = 0.862 and r = 0.914; all P ＜ 0.01 ).These increases were abolished by Y27632 pretreatment( P ＜0.01 ).CONCLUSION: rMIF increases the synthesis of collagen type Ⅲ in MRC - 5 cells and Rho - kinase regulates the MIF - induced synthesis of collagen, suggesting that MIF may play important roles in the pathogenesis of airway remodeling.关键词
巨噬细胞移动抑制因子/成纤维细胞/胶原/Rho激酶Key words
Macrophage migration inhibitory factor/ Fibroblasts/ Collagen/ Rho - kinase分类
医药卫生引用本文复制引用
陈培芬,罗雅玲,赖文岩,邢晓雯,骆子义,邱智辉..巨噬细胞移动抑制因子经Rho途径促进人肺成纤维细胞III型胶原合成[J].中国病理生理杂志,2011,27(6):1176-1179,4.基金项目
国家自然科学基金资助项目(No.30770936) （No.30770936）
