中国病理生理杂志2011，Vol.27Issue(6)：1210-1212,1217,4.DOI:10.3969/j.issn.1000-4718.2011.06.030
TNF-α对小鼠脑微血管内皮细胞RhoA活性的影响
A novel mechanism of RhoA activation induced by tumor necrosis factor-α in mouse cerebral microvascular endothelial cells
摘要
Abstract
AIM: To investigate the effects of tumor necrosis factor α ( TNF - α ) on RhoA activity in mouse cerebral microvascular endothelial cells.METHODS: The bEnd.3 cells, a mouse brain microvascular endothelial cell line, were cultured.RhoA activity was analyzed by pull - down assay 10 min, 30 min and 60 min after TNF - α treatment.Expression of RhoA protein was determined by Western blotting 1h, 3h, 6 h, 12h and 24 h after TNF - α treatment.Small interfering RNA ( siRNA ) targeting to p115RhoGEF or control nsRNA was transfected into bEnd.3 cells.The expression of p115RhoGEF was determined by Western blotting, and RhoA activity was detected by pull - down assay 30 min after TNF - α treatment.RESULTS: RhoA activity peaked at 30 min after TNF - α treatment P ＜0.01 ).TNF - α significantly increased the protein expression of RhoA at 12 h and 24 h ( P ＜0.05 ).Knock - down of p115RhoGEF by siRNA in bEnd.3 cells attenuated TNF - α - induced RhoA activation ( P ＜ 0.05 ).CONCLUSION: TNF - α up - regulates RhoA activity and expression.p115RhoGEF may play a role in TNF - α - induced activation of RhoA.关键词
肿瘤坏死因子/Rho/内皮细胞Key words
Tumor necrosis factor/ Rho/ Endothelial cells分类
医药卫生引用本文复制引用
邓小鹿,彭镜,何芳,杨丽芬,吴丽文,尹飞..TNF-α对小鼠脑微血管内皮细胞RhoA活性的影响[J].中国病理生理杂志,2011,27(6):1210-1212,1217,4.基金项目
国家自然科学基金资助项目(No.30872790 ) （No.30872790 ）
