军事医学2011，Vol.35Issue(6)：432-436,5.
实时RT-PCR检测甲型H3N2流感病毒R292K耐药位点
Development and evaluation of a novel real-time reverse-transcriptase PCR assay for detection of R292K mutation of the H3N2 influenza A virus
摘要
Abstract
Objective To develop a rapid and sensitive assay for detection of R292K oseltamivir-resisitant mutation of the H3N2 influenza A virus. Method Using specific Taq-MGB probe targeting R292K mutation, a real-time RT-PCR assay was developed. The sensitivity of the assay was analyzed using a lO-fold dilution series of plasmid DNA containing partial neuramidinase( NA ) sequence with R292K mutation. The specificity of the assay was also analyzed with H3N2 influenza virus without R292K mutation and other common respiratory viruses. Results and Conclusion The detection limit of the R292K rRT-PCR assay was 500 copies/μl. No cross-reaction with H3N2 virus without R292K mutation and other five respiratory viruses was observed. The R292 real-time RT-PCR assay is specific and sensitive , and potentially useful in influenza resistance surveillance and clinical diagnosis.关键词
流感病毒/H3N2亚型/抗病毒药物/耐药性/实时RT-PCRKey words
influenza A virus/ H3N2/ anti-influenza drugs/ drug-resistance/ real-time RT-PCR分类
医药卫生引用本文复制引用
刘娟,姜涛,徐莉娟,于曼,秦鄂德,秦成峰..实时RT-PCR检测甲型H3N2流感病毒R292K耐药位点[J].军事医学,2011,35(6):432-436,5.基金项目
"重大新药创制"科技重大专项资助项目(2009ZX10004-204,2008ZX10004-001) （2009ZX10004-204,2008ZX10004-001）
国家自然科学基金资助项目(81000722) （81000722）
