浙江医学2011，Vol.33Issue(7)：967-970,985,5.
白藜芦醇通过激活静息信号调节蛋白途径抑制自噬减弱心肌缺血再灌注损伤
Resveratrol protects myocardium against reperfusion injury by inhibiting autophagy through SIRT1 pathway
摘要
Abstract
Objective To investigate the effects of resveratrol on myocardial reperfusion injury and autophagy in rats.Method Thirty- two male Sprague- Dawley rats weighing 250- 300 g were randomly divided into four groups (n=8 each): group C (control); group R (resveratrol treatment); group RN (resveratrol + nicotinamide treatment) and group N (nicotinamide treatment).The left ventricular functional parameters were recorded at baseline, at 30min and 120min of reperfusion.The infarct size was measured after the heart was stained with triphenyltetrazolium chloride.The level of autophagy was determined by electron microscopy and Western Blot.Results The group R showed better functional parameters and smaller infarct size (16.6 ± 2.5％ vs 45 ± 3％) than the group C at 120min after reperfusion, while there were no differences between values of baseline and at 30min.The level of autophagy in group R was lower than that in group C.All the beneficial effects were abrogated by the SIRT1 inhibitor nicotinamide.Conclusion Resveratrol can protests myocardium from ischemia/reperfusion injury, and this protective effect may be associated with inhibition of autophagy via the SIRT1 pathway.关键词
白藜芦醇/缺血再灌注损伤/自噬/静息信号调节蛋白途径Key words
Resveratrol / Reperfusion injury / Autophagy / SIRT引用本文复制引用
杨礼,周海燕..白藜芦醇通过激活静息信号调节蛋白途径抑制自噬减弱心肌缺血再灌注损伤[J].浙江医学,2011,33(7):967-970,985,5.基金项目
浙江省科技厅课题(2008C23056) （2008C23056）
