中国普通外科杂志2011，Vol.20Issue(5)：491-493,3.
survivin siRNA对裸鼠乳腺癌细胞移植瘤微血管生成的影响
Effects of survivin siRNA on angiogenesis of human breast cancer xenografts in nude mice
摘要
Abstract
Objective To investigate the effects of survivin siRNA on angiogenesis of human breast cancer MCF-7 cell xenografts in nude mice. Methods The animal model of tumor xenograft was established by transplantation with MCF-7 cells into nude mice. The models were randomly assigned to 3 groups: PcDNA3-Survivin siRNA treatment, PcDNA treatment and control group, which were respectively injected with PcDNA3-siRNA plasmid, PcDNA3 empty plasmid or saline solution into the xenograft tumor after tumor formation in the mice. Survivin mRNA and protein levels were examined by RT-PCR and Western blot.Microvessel density was measured by immunohistochemistry staining. Results Survivin mRNA, protein levels and microvessel density in PcDNA3-Survivin siRNA treatment group were significantly decreased compared with PcDNA treatment and control group ( P ＜ 0.05 ); whereas no significant difference was noted between PcDNA treatment and control group (P ＞ 0. 05 ). Conclusions Survivin siRNA not only decreases survivin expression, but also inhibits angiogenesis in MCF-7 xenografts.关键词
乳腺肿瘤/病理学/Survivin/siRNA/裸鼠/血管生成Key words
Breast Neoplasms/pathol/ Survivin/ siRNA/ Nude Mice/ Angiogenesis分类
医药卫生引用本文复制引用
武彪,刘正人,李映良,涂发妹,刘东升,吴冬娣,吴玮斌..survivin siRNA对裸鼠乳腺癌细胞移植瘤微血管生成的影响[J].中国普通外科杂志,2011,20(5):491-493,3.基金项目
江西省卫生厅科技计划资助项目(20081053). （20081053）
