中国普通外科杂志2011，Vol.20Issue(5)：499-502,4.
小鼠异位小肠移植模型的建立
Establishment of heterotopic small bowel transplantation model in mice
李亭 1齐海智 1贺志军 1李一宁 1吴建国1
作者信息
- 1. 中南大学湘雅二医院普外移植科,湖南,长沙,410011
- 折叠
摘要
Abstract
Objective To investigate the surgical procedures for establishing a reliable murine model of heterotopic small bowel transplantation (SBTx) , with the purpose of providing a good experimental tool for the rejective reaction study in SBTx. Methods C57BL/6 mice were used as donor and the BALB/c mice used as recipient animals. The procedure of segmental heterotopic SBTx in allogenic mice was performed as following: the donor's portal vein was anastomosed end-to-side to the recipient's inferior vena cave (IVC) ,and then the donor's superior mesenteric artery (SMA) with an aortic patch was anastomosed end-to-side to the recipient abdominal aorta. Subsequently, proximal end of the graft intestine was ligated and the distant end was anastomosed end-to-side to the recipient's jejunum. The mice were fasted for 3 days, but free access to water after the operation. In addition, the mice were given 5 ％ glucose-saline solution (2 mL ) twice daily by subcutaneous injection, but without use of antibiotic and immunosuppressive therapy. The models survived beyond 5 days were considered to be successful. Results Segmental SBTx were perfomed in 30 mice, with a postoperative day (POD) 5 survival rate of 70 ％ (21/30). The operating time in donor was (41 ± 5.5 ) min, the graft warm ischemic time was about 0.5 min, the time for trimming the aortic patch in SMA was 3 min and cold preservation of donor' s small bowel was ( 30 ± 7.5 ) min. The time of recipient preparation and transplantation was ( 90 ± 7. 5 ) min, in which the abdominal aorta and IVC blockage time was (40 ± 3.0 ) min, the average time for the vein anastomosis and the arterial anastomosis was ( 10 ± 2.0 ) min and ( 15 ± 2.5 ) min, respectively. The average bleeding volume was 0.2 mL. The operation failed in 9 cases, due to arterial anastomotic thrombosis and anastomosis stenosis (6 cases ) ,massive hemorrhage (2 cases ) and intraperitoneal infection ( 1 case). Conclusions The high quality of the donor's small bowel segment harvesting, excellent vascular and intestinal anastomosis as well as adequate supplement of liquid to both donor and recipient are all crucial factors to ensure the efficiency and success of SBTx.关键词
小肠/移植/移植,异位/模型,动物/小鼠Key words
Intestine, Small / transpl/ Transplantation, Heterotopic/ Models, Animal/ Mouse分类
医药卫生引用本文复制引用
李亭,齐海智,贺志军,李一宁,吴建国..小鼠异位小肠移植模型的建立[J].中国普通外科杂志,2011,20(5):499-502,4.
