中国免疫学杂志2011，Vol.27Issue(8)：709-713,5.DOI:10.3969/j.issn.1000-484X.2011.08.007
玉屏风散对小鼠哮喘合并人偏肺病毒感染的干预机制探讨
Inhibition of the airway hyperresponsiveness and inflammation by Yu-Ping-Feng powers in an asthmatic mouse model infected with human metapneumovirus
摘要
Abstract
Objective:To investigate the impact of Yu-Ping-Feng power on the airway responsiveness and inflammatory in an asthmatic mouse model infected by human metapneum ovirus and the possible underlying mechanisms.Methods:Forty-eithtBALB/c mice were randomly divided into 6 groups.All the mice except control group and OVA group were inoculated intranasally with hMPV after underwent ovalbum in (OVA)sensitization and challenge,then being orally lavaged with various doses of Yu-Ping-Feng for 1 week.Airway responsiveness to inhaled methamholine was measured and bronchoalveolar lavage(BAL)was performed after the last challenge.Cells in BAL fluid(BALF)was counted and inflammatory characteristics of lungs was scored by staining with hematoxylin and eosin.Cytokines of lungs were detected by flow cytometry.Results:(1)OVA+hMPV group and low dose group displayed the highest airway responsiveness,significant difference was found between OVA group and OVA+hMPV group(P＜0.05).High dose group significantly reduced airway responsiveness,lower than OVA+hMPV group.(2)The numbers of total white cells and eosinophils in the BALF from OVA+hMPV group was significantly greater than control group,OVA group and high dose group(P＜0.05);(3)Histological score of peribronchiolitis,alveolitis and perivasculitis in all OVA-sensitized/challenged groups were significantly higher than that in control.OVA group and high dose group had significantly milder peribronchiolitis and alveolitis than OVA-hMPV group.(4) There was no difference in IFN-γlevel between control group and OVA group.IFN-γ,IFN-γ/IL-4 of each treatment group was significantly higher than that of OVA+hMPV group.IL-4 of All OVA-sensitized/challenged groups significantly higher than that of the control group(P＜0.01),but on significantly difference among the groups.IL-17 of OVA+hMPV group was significantly higher than that of OVA group,media dose group and high dose group.Conclusion:Yu-Ping-Feng can inhibit the airway hyperresponsiveness and airway inflammation of hMPV infection in asthmatic mice,and improve th Th1/Th2 in balance.关键词
玉屏风散/人偏肺病毒/哮喘/气道反应性Key words
Yu-Ping-Feng power/Human metapneum ovirus/Asthma/Airway responsiveness分类
医药卫生引用本文复制引用
李荣培,陈昕,刘平,陆彪,赵晓东..玉屏风散对小鼠哮喘合并人偏肺病毒感染的干预机制探讨[J].中国免疫学杂志,2011,27(8):709-713,5.基金项目
本文为国家自然科学基金重点项目基金(30730098)和重庆市杰出青年科学基金(CSCT,2008BA5040) （30730098）
