山东农业科学Issue(5)：1-3,7,4.
利用农杆菌介导法获得转cry2A抗虫基因水稻的研究
Development of Japonica Rice with Insect- resistant Gene Cry2A * by Agrobacterium - mediated Transformation
摘要
Abstract
Lepidopteran pests, especially stemborers and leaffolders, had obvious repulsionto Bacillus thuringiensis (BT) toxic protein encoded by cry2A* gene, so the pC -2A plasmid with cry2A * gene was transferred into the genome of rice variety Zhendao 88 by Agrobacterium - mediated method. Then the transgenic rice plants were obtained through Basta screening based on the tight linkage of cry2A* gene with Bastaresistant gene. The PCR and AGE analysis indicated that the cry2A * gene had been successfully integrated into Zhendao 88 genome. The field test showed that the transgenic plants had much better insect resistance compared with common plants.关键词
农杆菌介导法/cry2A基因/水稻/抗虫性Key words
Agrobacterium- mediated method/ cry2A* gene/ Rice/ Insect resistance分类
农业科技引用本文复制引用
吴振映,柳絮,王文英,姚方印,刘开启..利用农杆菌介导法获得转cry2A抗虫基因水稻的研究[J].山东农业科学,2011,(5):1-3,7,4.基金项目
国家转基因专项(2008ZX08001-001) （2008ZX08001-001）
