中国农业科学2011，Vol.44Issue(10)：1980-1988,9.DOI:10.3846/j.issn.0578-175.2011.10.002
大豆芽期耐盐和耐低温位点的遗传重叠
Genetic Overlap Between Salt and Low-Temperature Tolerance Loci at Germination Stage of Soybean
摘要
Abstract
[Objective]The main purpose of this paper was to map the QTLs related with salt tolerance and low-temperature tolerance at germination stage with backeross introgression lines (ILs), and analyze the genetic overlap between them.These overlapping QTL loci could be used to breed soybean varieties with both salt tolerance and low-temperature tolerance, improve efficiency of stress-tolerance breeding.[Method]A primary backcross introgression lines (ILs) were constructed with Hong-feng 11 as recurrent parent and Harosoy as donor parent.Then 48 individuals from BC2F4 introgression populations were obtained after screening in 1.75％ NaCl solution, and 40 individuals were screened out after 6℃ low-temperature treatment comparing with the Hong-feng11 at germination stage.The QTL identification of the germination stage was conducted by chi-square test and analysis of variances with the two introgression populations.[Result]There were 22 QTL located with BC2F4 introgression populations after salt stress, and 15 QTL were mapped after low-temperature stress.Seven overlapping QTLs between salt tolerance and low-temperature tolerance were detected on 6 linkage groups at germination stage.[Conclusion]In total, there were 31.81％ of salt tolerance and low-temperature tolerance loci existed genetic overlap.关键词
大豆/回交导入系/耐盐性/耐低温性/遗传重叠Key words
soybean/ introgression lines/ salt tolerance/ low-temperature tolerance/ genetic overlap引用本文复制引用
邱鹏程,陈庆山,张闻博,蒋洪蔚,刘春燕,李灿东,范冬梅,曾庆力,韩冬伟,胡国华..大豆芽期耐盐和耐低温位点的遗传重叠[J].中国农业科学,2011,44(10):1980-1988,9.基金项目
国家转基因专项(2009ZX08009-013B)、国家"948"项目(2006-G1A) （2009ZX08009-013B）
