中国农业科学2011，Vol.44Issue(10)：2022-2028,7.DOI:10.3846/j.issn.0578-175.2011.10.007
粗山羊草Y192中抗叶锈基因Lr Y192的SSR定位
SSR Mapping of Leaf Rust Resistance Gene LrY192 in Aegilops tauschii Y192
摘要
Abstract
[Objective] The objective of this study is to find new leaf rust resistance gene in Aegilops tauschii, and provide valuable information and germplasm for wheat leaf rust resistance breeding. [Method] Hybridization between Ae. tauschii Y192 (resistant) and Ae. tauschii Y2272 (susceptible) was carried out and the resistance of F2 population was evaluated by inoculated Puccinia triticina for investigating the resistance genes in Y192. Bulk segregation analysis and microsatellite markers on chromosome D were used to tag the resistance gene in Y192. The genetic distance was calculated by MapChart software. Gene postulation and MAS were also used to identify the resistance genes in Y192. [Result] F1 population derived from the crossing Y192 and Y2272 were resistant, and the ratio of resistance/susceptible was 3:1 in F2 generation. This indicated that a dominant wheat leaf rust resistance gene was presented in Y192. The gene was temporarily designated as LrY192. Three microsatellite markers Wmc245, Xgwm296 and Xgwm261 were acquired from SSR markers and linked to LrY192 with genetic distance of 4.1, 18.9, and 26.2 cM, respectively. According to the locations of the linked markers, the resistance gene was located on chromosome 2D.[ Conclusion ] Based on the chromosomal location and the resistance pattern of the gene, it is concluded that LrY192 is a novel leaf rust resistance gene, and could be selected by Wmc245.关键词
粗山羊草/小麦叶锈病/抗病基因/SSRKey words
Aegilops tauschii/ wheat leaf rust/ resistance gene/ SSR引用本文复制引用
胡亚亚,冯丽娜,冀红柳,孙一,张娜,魏学军,杨文香,刘大群,贾继增..粗山羊草Y192中抗叶锈基因Lr Y192的SSR定位[J].中国农业科学,2011,44(10):2022-2028,7.基金项目
国家公益性行业(农业)科研号项项目(200903035)、教育部博士点新教师基金(20101302120005) （农业）
