江苏大学学报（医学版）2011，Vol.21Issue(4)：281-284,288,5.
银杏内酯B对大鼠胚胎中脑神经细胞生长的影响
Effects of ginkgolide B on the development of embryonic rat midbrain neurons
谭才宏 1缪丽燕2
作者信息
- 1. 江苏大学附属医院药剂科,江苏,镇江,212001
- 2. 苏州大学附属第一医院药剂科,江苏,苏州,215006
- 折叠
摘要
Abstract
Objective : To establish a hypoxanthine guanine phosphorribosyl- transferase ( HGPRT ) deficient EL/4 cell line for setting up mouse T cell subset hybridana. Methods: After treated with ethylmethanesulfonate ( EMS) , the EL/4 cells were selected by8-Azaguanine ( 8-AG) forn lower concentration ( 5 μ/ml ) to higher concentration ( 80 μg/ml ) step by step. The mRNA expression of HGPRT of the resistant cells was detected by RT-PC.R In the encd, 8-AG selected EL/4 cells were tested for fusing with CD4+ T lymphocytes isolated from mouse spleen by Dynabeads Result: The selected EL/4 cells could resistant with higher concentration ( 80 μ/ml) of8-AG and maintained in20 μg/m18-AG medium, and couldn't survive with in hypoxanthine-am inop terin- thymidine ( HTA) medium. The result of RT-PCR revealed that there was no mRNA expression of HGPRT gene can be detected in these8-AG resistant cells, and the cell line was named asmutant EL/4 cells( mEL/4) . Furthermore, FCM analysis shown thatmEL/4 cells could be fused with the CD4+ T lymphocytes isolated from mouse spleen. Conclusion : The HGPRT deficient El/4 cell line ( mEL/4) was established successfully, it will provide useful cell the for establishtig T cell hybndana.关键词
银杏内酯B/神经生长因子/海人藻酸/大鼠胚胎中脑神经细胞Key words
ethylmethanesulfonate/ 8-Azaguanine/ HGPRT/ mutation/ EL/4分类
医药卫生引用本文复制引用
谭才宏,缪丽燕..银杏内酯B对大鼠胚胎中脑神经细胞生长的影响[J].江苏大学学报（医学版）,2011,21(4):281-284,288,5.
