安徽中医学院学报2011，Vol.30Issue(4)：51-54,4.DOI:10.3969/j.issn.1000-2219.2011.04.018
丹参联合5-氟尿嘧啶腹腔注射对大鼠胃肿瘤及其相关蛋白表达的影响
Effects of Radix Salvia Miltiorrhiza Combined with Intraperitoneal Injection of 5-Fluorouracil on Implanted Gastric Tumor and Expression of Gastric Tumor Related Proteins in Rats
摘要
Abstract
Objective To explore the mechanism of Radix Salviae miltiorrhizae (RSM) enhancing antineoplasm effects of 5-fluorouracil (5-Fu) against gastric tumor. Methods The rat model of implanted gastric tumor was induced using Walker-256 cell line. Sixty male rats with gastric tumor were randomized into three groups, namely, normal saline (NS) treated group, 5-Fu treated group, and 5-Fu combined with RSM treated group. Seven days after the implantation of gastric tumor cells, each rat was daily given intraperitoneal injection of NS 40 ml/kg in NS treated group, 5-Fu 20 ml/kg in 5-Fu treated group, and 5-Fu 20 ml/kg plus RSM 60 mg/kg in 5-Fu combined with RSM treated group, respectively, during 5 successive days. After the chemotherapy, the general conditions were observed in rats with implanted gastric tumor of the three groups. The inhibition rates of gastric tumor and the prolongation rates of survival time were compared among the three groups. The expression levels of P53, CD44, H-ras, and nm23 proteins were assayed using immunohistochemistry method. Results (1) Two weeks after the implantation of gastric tumor cells, rats in NS treated group manifestated such symptoms as loss of appetite, decrement of activities, slow response to outside stimuli, gradual emaciation, and even ascites. While in 5-Fu treated group, and 5-Fu combined with RSM treated group, above-mentioned manifestations gradually appeared about 4 weeks after the implantation of gastric tumor cells. (2) Compared with those in NS treated group, the sizes of implanted gastric tumor were significantly decreased in 5-Fu treated group, and 5-Fu combined with RSM treated group. The sizes of implanted gastric tumor and the inhibition rates of tumor size were significantly lower in 5-Fu combined with RSM treated group than those in 5-Fu treated group. (3)The survival time in 5-Fu treated group, and 5-Fu combined with RSM treated group was significantly longer than that in NS treated group; compared with 5-Fu treated group, the survival time was significantly prolonged, and the prolongation rates of survival time were significantly increased in 5-Fu combined with RSM treated group. (4)Compared with those in NS treated group, the positive expression levels of H-ras protein were significantly decreased, and the positive expression levels of nm23 protein were significantly increased in 5Fu combined with RSM treated group. Other indicators showed an increasing or decreasing tendency with no significant differences among the three groups. Conclusion RSM can enhance the antineoplasm efficacy of 5-Fu against implanted gastric tumor, the mechanism might be related with inhibiting the expression of oncoprotein P53 , H-ras, and CD44 while increasing the expression of tumor suppressor protein nm23.关键词
胃肿瘤/丹参/5-氟尿嘧啶/P53、CD44、H-ras/nm23Key words
gastric tumour/ Radix Salviae miltiorrhizae/ 5-fluorouracil/ P53/ CD44/ H-ras/ nm23分类
医药卫生引用本文复制引用
于庆生,王园,潘晋方,王汉明,帅剑锋,张琦,刘举达..丹参联合5-氟尿嘧啶腹腔注射对大鼠胃肿瘤及其相关蛋白表达的影响[J].安徽中医学院学报,2011,30(4):51-54,4.基金项目
安徽省科技攻关计划项目(07010300201) （07010300201）
安徽省人才基金(2006Z040) （2006Z040）
