安徽中医学院学报2011，Vol.30Issue(4)：63-67,5.DOI:10.3969/j.issn.1000-2219.2011.04.021
复方银杏丹参颗粒对H9c2细胞氧化应激损伤的保护作用
Protective Effects of Compound Yinxing Danshen Granules against Oxidative Stress Damage in H9c2 Cells
曹康 1张婷婷 1薛雪1
作者信息
- 1. 安徽中医学院第一附属医院药学部,安徽,合肥,230031
- 折叠
摘要
Abstract
Objective To observe the protective effects of Compound Yinxing Danshen Granules (CYDG) , a traditional Chinese medicine prescription for activating blood circulation and removing blood stasis and obstacles from the collaterals, and relieving pain, against oxidative stress damage in rat H9c2 cells in vitro. Methods Hydrogen peroxide (H2 O2) was used as exogenous free radical generating system to induce the lipid peroxidation damage of cardiac muscle cells. The protective effects of CYDG against H2 O2 -induced damage on cardiac muscle cells were observed. Results The method of methyl thiazolyl tetrazolium (MTT) staining showed that CYDG significantly increased the rate of cell viability. CYDG could increase the activities of catalase and superoxide dismutase, and decrease the levels of lactate dehydrogenase and malondialdehyde in in vitro cultured H9c2 cells. dichlorofluorescein-diacetate (DCFH) labeling showed that CYDG increased the levels of reactive oxygen species (ROS) in H2 O2-stressed H9c2 cells. Western blot showed that CYDG increased the expression of heme oxygenase-l (HO-1) in H2O2-stressed H9c2 cells. Conclusion CYDG could protect H9c2 myocardial cells from the damage induced by H2O2-oxidative stress, and increase the antioxidant capacity of cardiac muscle cells.关键词
复方银杏丹参颗粒/H9c2细胞/过氧化氢/氧化应激损伤/血红素加氧酶-1Key words
Compound Yinxing Danshen Graunles/ H9c2 cells/ hydrogen peroxide/ oxidative stress dam-age/ haem oxygenase-1分类
中医学引用本文复制引用
曹康,张婷婷,薛雪..复方银杏丹参颗粒对H9c2细胞氧化应激损伤的保护作用[J].安徽中医学院学报,2011,30(4):63-67,5.
