江苏大学学报（医学版）2011，Vol.21Issue(4)：289-293,5.
Hsa-mir-216a真核表达载体的构建及其在胃癌细胞SGC7901/DDP中的表达
Construction of hsa-mir-216a eukaryotic expression vector and its expression in gastric cancer cells SGC7901/DDP
摘要
Abstract
Objective: To observe the protective effects of puerarin on cardiac muscle in diabetic rats and explore the mechanism of myocardial fibrosis. Methods: Diabetic model rats were established by intraabdominal injection of streptozotocin. Puerarin- treated rats were administered by gastric canal at different dose of puerarin respectively. After 12 weeks, blood sugar and heart weight index were examined in all groups. The structure of heart and the change of collagen fiber were examined by lightmicroscope. Semiquantitative expressions of MMP-2 and MMP-9 protein were determined by immunohistochem istry and Westem Blot. Results : Fasting blood glucose, heart weight index of the diabetic rats were significantly up- regulated ( P ＜ 0. 05 ) , collagen fiber were much higher( P ＜ 0. 05 ) , the protein expression of MMP-9 were significantly up- regulated( P ＜ 0. 05 ) , MMP-2 were significantly decreased. Compared with the diabetic group,fasting blood glucose and heart weight index of puerarin therapy groups were significantly decreased( P ＜0. 05 ) , collagen fiber were declined significantly( P ＜ 0. 05 ) , the protein expression of MMP-9 were decreased and MMP-2 were up-regulated( P ＜0. 05 ) . Conclusion : The expression of MMP-2 and MMP-9 on cardiac muscle are related to myocardial fibrosis of diabetic, puerarin has therapeutic effect on diabetic cardiomyopathy through affecting the changes of above mentioned indexes.关键词
mir-216a/真核表达载体/SGC7901/DDP细胞/转染Key words
puerarin/ diabetic/ myocardial fibrosis/ matrix metalloproteinases-2 / matrix metalloproteinases-9/ rat分类
医药卫生引用本文复制引用
徐红美,许文林,姚俊,冷加燕,吴晓君,陈巧云..Hsa-mir-216a真核表达载体的构建及其在胃癌细胞SGC7901/DDP中的表达[J].江苏大学学报（医学版）,2011,21(4):289-293,5.基金项目
国家自然科学基金资助项目(81070423) （81070423）
江苏省卫生重大课题基金资助项目(K2005017) （K2005017）
