江苏大学学报（医学版）2011，Vol.21Issue(4)：303-307,封2,6.
YB-1腺病毒载体的构建及其对乳腺癌MCF-7细胞耐药性的影响
Construction of hYB1 gene recombinant adenovirus vector and its increasing in the drug resistance of breast cancer MCF-7 cells strain
摘要
Abstract
Objective: To investigate the effect of cysteine- rich61( Cyr61) on the chemoresistance of human pancreatic cancer cells induced by gemeitabine. Methods: Human pancreatic cancer cells MIA PaCa2 were cultured and infected with empty vector vector expression Cyr61 or shRNA. The ability of cell proliferation was analyzed by clone formation assay; the apoptosis was evaluated by the flow cytometry analysis stained with propidium iodide. The expression of the apoptosis related proteins were examined by Westem blot assay.Results: As shown in clone formation assay, Cyr61 enhanced the antiproliferation capability of MIA PaCa2 to gemcitabine( P ＜0. 05 ) . Compared with control group, theM MIA PaCa2 forced expression of Cyr61 had less apoptosis rate( P ＜0. 05). On the contrary, knockdown the endogenous Cy61 inhited the antiproliferation capability to gemcitabine as well as enhanced the apoptosis rate ( P ＜ 0. 05). Western blot shown that there were increased Bcl-2 expression and decreased caspase-2 ,8 and cleaved PARP expression in Cyr61 forced expression group compared with control groups, while there were decreased Bcl-2 expression and increased caspase-2,8 and cleaved PARP expression in MIA PaCa2/Cyr61 i group. Conclusions: Cyr61 endowed the chemoresistance ability of pancreatic cancer cell to gencitabine through inhibiting the pathway.关键词
hYB1基因/MCF-7细胞/腺病毒载体/耐药Key words
pancreatic carcinoma/ cysteine- rich61/ gemcitabine/ apoptosis分类
医药卫生引用本文复制引用
冷加燕,许文林,沈慧玲,龙璐璐,吴晓君,陈巧云,姚俊..YB-1腺病毒载体的构建及其对乳腺癌MCF-7细胞耐药性的影响[J].江苏大学学报（医学版）,2011,21(4):303-307,封2,6.基金项目
国家自然科学基金资助项目(81070423) （81070423）
江苏省自然科学基金资助项目(BK2010332) （BK2010332）
江苏省卫生厅重大资助项目(K2005017) （K2005017）
