针灸临床杂志2011，Vol.27Issue(5)：57-59,3.
电针对大鼠脊髓损伤后细胞凋亡PARP-1全长影响的研究
The Effect of Electro-acupancture on PARP-1 of Cell Apoptosis after Spinal Cord Injury of Rats
摘要
Abstract
Objective:With the model rats of acute SCI, the paper investigates that the electro - acupuncture affects the expression of neural cell apoptosis and changes related genes for PARP - 1 after spinal cord injury of rats. The paper tries to show that the mechanisms of electro - acupuncture on spinal cord injure and the study of related important factors of mitochondrion excitement apoptosis way. Methods: Acute spinal cord injury models by Allen's preparation, the rats were divided into acupuncture group and drug group (Caspase -3 inhibitor group), model group, sham operation group. Apoptotic cells were marked by Tunel method. Results:Tunel labeling results showed that spinal cord injury after 6h, PARP - 1 expression in the model group appeared, 1 d expression was the highest,on the 3 d,7 d it gradually decreased, on the 14 d it was the lowest. Model group expression levels were higher than that in sham operation group and drug group on the 1 d, 3 d ( P ＜ 0. 05 ). Expression of electro - acupuncture group was lower than that in model group on the 3 d (P ＜ 0. 05 ). Conclusion:First: Apoptosis occurs in rats after acute spinal cord injury. Spinal cord injury is the main pathological mechanism. Second: The expression of PARP - 1 in rat neurons increased gradually after spinal cord injury, suggesting that PARP - 1 may be involved in spinal cord injury. Third: Electro - acupuncture inhibits apoptosis of neural cells and reduces spinal cord injury by inhibiting neurons expression of PARP - 1 in spinal cord injury.关键词
电针/脊髓损伤/细胞凋亡/TUNEL/PARP-1全长Key words
Electro - acupuncture/ Spinal cord injury/ Cell apoptosis/ TUNEL/ PARP - 1分类
医药卫生引用本文复制引用
李晓宁,王宁..电针对大鼠脊髓损伤后细胞凋亡PARP-1全长影响的研究[J].针灸临床杂志,2011,27(5):57-59,3.基金项目
黑龙江省教育厅科学技术研究项目,编号:11531343 （）
黑龙江省中医药、中西医结合重点科研项目,编号:ZHV08-Z42. （）
