中国农业科学2011，Vol.44Issue(10)：2139-2145,7.DOI:10.3846/j.issn.0578-175.2011.10.019
17beta-雌二醇通过beta受体和cAMP-ERK1/2级联调节仔猪睾丸支持细胞cyclinA2mRNA的表达
17-beta Estradiol Regulates the Expression of CyclinA2 mRNA of Cultured Immature Boar Sertoli Cells via Estrogen Receptor beta, cAMP-PKA and ERK1/2
摘要
Abstract
[Objective]The objective of this study was to identify whether 17beta-estradiol regulates the expression of eCyclinA2 mRNA via the estrogen receptor beta (ERbetβa) and the cAMP-PKA-extracellular signal-regulated kinase (ERK1/2)pathway.[Method]Cultured immature boar sertoli cells were treated with 10-9 mol·L-1 17beta-estradiol, and real-time PCR was used to detect the expression of cyclinA2 mRNA.[Result]Treatment with 17beta-estradiol increased the expression of cyclinA2 mRNA from 15 min to 90 min (P＜0.05).The effects of 17beta-estradiol activity peaked at 30 min compared to the control cells (P＜0.05).Combined treatment with ICI182780 and ERβ reduced the 17beta-estradiol-induced increase in the expression of cyclinA2 mRNA (P ＜0.05), but ERβ alone did not significantly affect these parameters (P＜0.05).Both 17beta-estradiol and forskolin induced the abundance of cyclinA2 mRNA (P＜0.05 for both).Combined treatment with Rp-cAMP, H-89 and U0126 reduced the 17beta -estradiol-induced expression of cyclinA2 mRNA (P ＜0.05 for all), but Rp-cAMP, H-89 and U0126 alone had no significant effect on the abundance of cyclinA2 mRNA, compared to the control group.[Conclusion]This study showed that 17beta-estradiol regulates the expression of cyclinA2 mRNA via the activation of ERβ, cAMP-PKA and ERK1/2.关键词
17beta-雌二醇/睾丸支持细胞/细胞周期素A2/雌激素受体/细胞外信号调节的蛋白激酶1/2Key words
17beta-estradiol/ sertoli cell/ cyclinA2/ estrogen receptor/ ERK1/2引用本文复制引用
左敬,甘瑞,张国升,张姣姣,朱峰伟,孙燕,王鲜忠,张家骅..17beta-雌二醇通过beta受体和cAMP-ERK1/2级联调节仔猪睾丸支持细胞cyclinA2mRNA的表达[J].中国农业科学,2011,44(10):2139-2145,7.基金项目
国家自然科学基金项目(31072183)、中央高校基本科研业务费专项项目(XDJK20098035) （31072183）
