中国卒中杂志2011，Vol.6Issue(5)：388-394,7.
皮层电刺激联合康复锻炼对大鼠局灶性脑缺血模型前肢运动功能及运动区突触可塑性相关蛋白表达的影响
Cortical electrical Stimulation Combined with Rehabilitative Training Enhance Forelimb Motor Function and Synaptic Plasticity Following Focal Cortical Ischemia in Rats
摘要
Abstract
Objective To assess the behavioral and synaptic plasticity effects of combining epidural cortical electrical stimulation with motor skills training following unilateral sensorimotor cortex (SMC)lesions in adult male rats.Methods Prior to lesion/electrode implantation surgeries, rats were pre-trained on the 'single pellet retrieval task' to a minimum criterion of 30％ success rate for two consecutive days. Then these rats received partial unilateral SMC lesions and implantation of electrodes over the remaining SMC. Fourteen days later, rats received daily reach training concurrent with anodal or cathodal 100 Hz or no stimulation for 14 days. Performance was measured as the percent of successes out of the total number of reach attempts [(total successes/total reach attempts)*100]. Conventional avidin biotinylated enzyme complex (ABC) immunohistochemical method was used quantify the expression and distribution of microtubule-associated protein 2 (MAP-2) and growth associated protein 43 (GAP-43) in motor cortical area underlying the electrode.Results There was no statistical significance between the two groups on the 14th day of preoperative training (P=0.546). The stimulation group had significantly greater rates of improvement with the impaired forelimb in comparison to control group (49.12％ vs 21.67％, P=0.004). The expression and distribution of MAP-2 and GAP-43 in the stimulating group were better than those in control group (GAP-43 : 0.3338 vs 0.3056. P=0.008; MAP-2: 0.4825 vs 0.4327. P=0.027).Conclusion These data indicate that cortical stimulation greatly improves the efficacy of rehabilitative reach training following SMC damage and raise the possibility that CS-induced functional improvements may be mediated by promoting the expression of MAP-2 and GAP-43 in perilesion cortex. and thus improve synaptic plasticity in cerebral ischemic rats.关键词
脑缺血/经皮神经电刺激/康复/神经元可塑性/微管相关蛋白质/GAP-45蛋白Key words
Brain ischemia/ Transcutaneous electric nerve stimulation/ Rehabilitation/Neuronal plasticity/ Microtubule-associated proteins/ GAP-43 protein引用本文复制引用
郑建,杨力军,谢瑞禄,赵兰峰,薛晓伟,王硕,赵继宗,曹勇..皮层电刺激联合康复锻炼对大鼠局灶性脑缺血模型前肢运动功能及运动区突触可塑性相关蛋白表达的影响[J].中国卒中杂志,2011,6(5):388-394,7.基金项目
国家自然科学基金面上项目:皮层电刺激联合康复锻炼对大鼠脑缺血模型运动区突触可塑性的作用及机制(30973112) （30973112）
