重庆医学2011，Vol.40Issue(20)：1982-1984,1987,4.DOI:10.3969/j.issn.1671-8348.2011.20.003
川芎嗪对人卵巢癌顺铂耐药细胞株COC1/DDP的逆转作用研究
Reversal effect of tetramethylpyrazine on cisplatin-resistant ovarian cancer cell line COC1/DDP
摘要
Abstract
Objective To establish a cisplatin-resistant ovarian cancer cell line COC1/DDP, and observe the reversal effect and mechanism of tetramethylpyrazine on COC1/DDP cell.Methods COC1/DDP cell line was established by Gradually increasing the concentration of cisplatin and intermittentinduced method to COC1 cell line in vitro.Reversal effect of tetramethyipyrazine on COC1/DDP cell proliferation was detected by Cell Counting Kit-8.The level of GSH was determined by GSH and GSSG Kit.The content of cisplatin was detected by HPLC.Results COC1/DDP was developed after 5 month,with stable resistance to cisplatin,not only resistance index was 9.44 , but also exhibited cross-resistance to carboplatin, vincristine and adriamycin at different degree.Tetramethylpyrazine 0.25mg/mL displayed the reversal effect on COC1/DDP cell line and the reversal fold was 3.76.Compared to the COC1 cell line,the level of GSH increased but the cispiatin content decreased in COC1/DDP cells, however,after the intervention of tetramethylpyrazine 0.25mg/mL,the level of GSH decreased while the cisplatin content increased in COCI/DDP cell(P＜0.01).Conclusion Tetramethylpyrazine exhibite the reversal effect on COC1/DDP,its mechanism may be relate to increasing the content of cisplatin by effecting on GSH/GST-π detoxification systems in COC1/DDP cells.关键词
川芎嗪/顺铂/COC1/DDP/多药耐药性Key words
tetramethylpyrazine / cisplatin / COC1/DDP / MDR引用本文复制引用
刘明华,任美萍,李蓉,章卓,肖顺汉..川芎嗪对人卵巢癌顺铂耐药细胞株COC1/DDP的逆转作用研究[J].重庆医学,2011,40(20):1982-1984,1987,4.基金项目
四川省卫生厅科学研究基金资助项目(060058). （060058）
