摘要
Abstract
Objective To investigate the expression of CD59- on the surface of granulocytic cells in patients with PNH and other type anemia,and to evaluate the diagnostic value of CD59 deficient expression.Methods Using PE fluorescence labeled monoclonal antibodies against CD59 ,the expression levels of CD59- were detected by flow cytometric analysis on granulocytic cells of 16 patients with PNH,7 with AA-PNH,33 with AA,29 with MDS.11 with MM,10 with SLF,10 with ALL,11 with AML,28 with MA, 66 with IDA and 13 with AIHA. One-way analysis of variance test was performed to compare the difference in the frequency of CD59 negative granulocytic populations.Results The average percents of CD59-cells expressed in granulocytes were 1.9％ in normal individuals,43.6％ in PNH, 29.3％ in AA-PNH,17.7％ in AA, 14.6％ in MDS, 12.1％ in MM, 12.1％ in SLF, 11.7％ in ALL,33.6％ in AML,11.6％ in MA , 8.0％ in IDA and 11.2％ in AIHA.Compared to the normal control group, the percentage of CD59- deficency increased apparently in patients with PNH , AA-PNH , AA, MDS, MM , SLE, ALL , AML, MA , IDA and AIHA ,and were statistics significant(P＜0.01 or P＜0.05).Conclusion The expression levels of CD59-deficency on granulocytic cells in patients with different type anemia are increase to different extent and the diminished expression of the surface molecular CD59 may not be specific to PNH diagnosis.关键词
贫血/流式细胞术/阵发性睡眠性血红蛋白尿症/CD59Key words
anemia / flow cytometry/ paroxysmal nocturnal hemoglobinuria / CD59
