分析测试学报2011，Vol.30Issue(4)：453-456,4.DOI:10.3969/j.issn.1004-4957.2011.04.020
微流控芯片测定盐酸金刚烷胺片中的盐酸金刚烷胺
Determination of Amantadine Hydrochloride in Amantadine Hydrochloride Tablets by Microfluidic Chip Method
摘要
Abstract
A new method of microfluidic chip with contactless conductivity detection was established for the determination of amantadine hydrochloride in amantadine hydrochloride tablets. The electrophoretic parameters, such as the variety and concentration of buffer solution and additive, separation voltage and injection time etc, were investigated. Under the optimal conditions, using 1 mmol/L HAc + 2 mmol/L NaAc ( pH 4. 5 ) + 0. 1 mmol/L SDS as buffer solution system, amantadine hydrochloride was separated and detected in 1 min at the separation voltage of 2.00 kV and 10 s injection time. The linear dependence of the concentration of amantadine hydrochloride ranged from 10 mg/L to 120 mg/L (r =0. 999 0), with a detection limit(S/N= 3) of 0. 6 mg/L. The spiked recoveries ranged from 97％ to 98％ with RSDs of 0. 8％ -2.2％. The method was simple, rapid and well reproducible, and could be used for the rapid detection and the quality control of the product.关键词
微流控芯片/非接触电导检测/盐酸金刚烷胺/测定Key words
microfluidic chip/ contactless conductivity detection/ amantadine hydrochloride/ determination分类
化学化工引用本文复制引用
蔡自由,李永冲,童艳丽,陈缵光..微流控芯片测定盐酸金刚烷胺片中的盐酸金刚烷胺[J].分析测试学报,2011,30(4):453-456,4.基金项目
国家自然科学基金资助项目(20575080和20727006) （20575080和20727006）
