分析测试学报2011，Vol.30Issue(4)：457-460,4.DOI:10.3969/j.issn.1004-4957.2011.04.021
偏最小二乘-分光光度法同时测定芦丁与槲皮素的方法研究
Simultaneous Determination of Rutin and Quercetin by Partial Least Squares - Spectrophotometry
摘要
Abstract
The maximum absorption wavelengths of rutin and quercetin are very close and their absorption spectra are seriously overlapping, so it's very difficult to determine individually rutin or quercetin by spectrophotometry without prior separation. In this paper, a partial least squares -spectrophotometry was developed for the simultaneous determination of rutin and quercetin. The absorption spectra of rutin and quercetin mixed standard were studied in the range of 200-500 nm and the absorbance values were recorded every 2 nm. A partial least-squares (PLS) model was built for the simultaneous determination of the contents of rutin and quercetin in the flos sophorae and synthetic sampies. The results indicated that the recoveries of rutin and quercetin in the flos sophorae were in the range of 95.6％ -99.8％ and 99.8％ -99. 9％ , respectively, and the recoveries of rutin and quercetin in the synthetic samples were in the range of 97.6％ - 102. 1％ and 100.2％ - 102.2％ , respectively. The method showed high accuracy and satisfied precision, and could reduce the effect of noise effectively.关键词
偏最小二乘-分光光度法/芦丁/槲皮素/同时测定Key words
partial least square spectrophotometry/ rutin/ quercetin/ simultaneous determination分类
数理科学引用本文复制引用
尚永辉,李华,孙家娟,王晓玲,耿薇..偏最小二乘-分光光度法同时测定芦丁与槲皮素的方法研究[J].分析测试学报,2011,30(4):457-460,4.基金项目
国家自然科学基金资助项目(20975081) （20975081）
西北大学研究生交叉学科资助项目(07YJC09) （07YJC09）
陕西省教育厅科研计划资助项目(2010JK899) （2010JK899）
咸阳师范学院专项科研基金资助项目(10XSYK205) （10XSYK205）
教育部科学技术研究重点资助项目(204182) （204182）
