中国药理学通报2011，Vol.27Issue(8)：1091-1095,5.DOI:10.3969/j.issn.1001-1978.2011.08.014
全反式维甲酸对骨肉瘤细胞143B生长的影响
Effect of ATRA on the growth of 143B osteosarcoma cells
摘要
Abstract
Aim To study the inhibitory effect of alltrans retinoic acid ( ATRA ) on 143B osteosarcomacells and the possible mechanism. Methods First,the endogenous retinoids nuclear receptor expression in143B and MC63 osteosarcoma cells were tested, Thencell counting was employed to test the proliferation in-hibitory effect of ATRA on 143B cells. Western blotwas used to test the caspase-3 level. as well as apopto-sis staining, to make sure that ATAR can not only in-hibits proliferation but also induces apoptosis in 143Bcells. Finally, the luciferase reporter assay was adopt-ed to check the osteogenic transcription factor activityof Runx2, the protein expression of osteopontin and osteocalcin were measured with western blot. Results Different types of retinoids nuclear receptor were detected in 143B and MG63 osteosarcoma cells. ATRA inhibited the proliferation of 143B cells in a concentration-dependent manner. The caspase-3 increased apparently after the cells were treated with ATRA for 12 hour and the apoptosis staining was positive apparently. The transcription activity of Runx2 increased, so did the protein level of osteopontin and osteocalcin.Conclusion ATRA can inhibit the proliferation of 143B osteosarcoma cells and induce the cell apoptosis.These effects may at least result from the osteogenic differentiation of osteosarcoma cells initialized by ATRA via retinoic acid signaling.关键词
全反式维甲酸/骨肉瘤/增殖抑制/凋亡/成骨分化/维甲酸信号Key words
all trans retinoic acid/ osteosarcoma/ proliferation inhibiting/ apoptosis/ osteogenic differentiation/ retinoic acid signaling分类
医药卫生引用本文复制引用
牟钰钦,周龙洋,杨秋珺,周岐新,何百成..全反式维甲酸对骨肉瘤细胞143B生长的影响[J].中国药理学通报,2011,27(8):1091-1095,5.基金项目
国家自然科学基金资助项目(No 81071462) （No 81071462）
