中国药理学通报2011，Vol.27Issue(8)：1098-1103,6.DOI:10.3969/j.issn.1001-1978.2011.08.016
海参岩藻聚糖硫酸酯抑制小鼠肿瘤生长和转移及其作用机制的研究
Anti-metastasis effect of SC-FUC on spontaneous metastasis of mouse Lewis lung carcinoma and its mechanism
摘要
Abstract
Aim To explore the inhibitory effect of sea cucumber fucoidan( SC-FUC ) on growth and metastasis of Lewis lung carcinoma in mice. Methods The Lewis lung carcinoma models of C57BL/6J mice were established. Different treatments were served from day 2 after transplantation and all mice killed after 21 days.The subcutaneous tumors were weighed to calculate inhibitory rate. The metastatic tumor foci on lung surface in mice were counted to calculate occurrence rate and inhibition rate of metastases on lung surface. The expressions of HIF-1α, VECF. HPA. MMP-2. MMP-9 mRNA were detected by RT-PCR. Results The tumor growth was suppressed significantly in SC-FUC low and high groups, the average inhibition rate was 45. 5％ compared with control group. The metastatic foci occurrence rate of the SC-FUC group was lower than control group, and the difference was significant.The SC-FUC could down-regulate the expression levels of HIF-1α, VECF, HPA. MMP-2, MMP-9 mRNA more than control. Conclusion SC-FUC has inhibitory effect on growth and metastasis of Lewis lung carcinoma cell in mice, which may be related to the inhibition of solid tumor angiogenesis and the reduction of migrated capacity of the Lewis lung carcinoma cell in vitro .关键词
海参岩藻聚糖硫酸酯/美国肉参/Lewis肺癌/HIF-1α/HPA/VEGFKey words
sea cucumber fucoidan/ Isostichopu.s badionotus/ Lewis lung carcinoma/ HIF-1α/ HPA/VECF分类
医药卫生引用本文复制引用
张珣,王静凤,杨玉红,常耀光,薛长湖..海参岩藻聚糖硫酸酯抑制小鼠肿瘤生长和转移及其作用机制的研究[J].中国药理学通报,2011,27(8):1098-1103,6.基金项目
国家自然科学基金资助项目(No 30972284) （No 30972284）
海洋公益性行业科研专项资助项目(No 201105029) （No 201105029）
国际科技合作项目资助项目(No 2010DFA31330) （No 2010DFA31330）
"泰山学者"建设工程专项经费 （）
