中国药理学通报2011，Vol.27Issue(8)：1126-1130,5.DOI:10.3969/j.issn.1001-1978.2011.08.022
帕金森病小鼠和大鼠模型黑质纹状体中胆绿素还原酶B的表达
Expression of biliverdin-Ⅸβ reductase in the nigrostriatal system in mouse and rat models of Parkinson's disease
摘要
Abstract
Aim To investigate the expression of biliverdin-Ⅸβ reductase( BVRB ) in the mouse MPTP model and rat 6-OHDA model of PD. Methods High-performance liquid chromatography ( HPLC ) was used to determine striatal levels of dopamine and its metabolites, and Western blot analysis to detect tyrosine hydroxylase ( TH ) expression in nigrostriatal tissue was used to confirm successful establishment of PD models. BVRB immunoreactivity in nigrostriatal tissue was investigated through Western blot analysis. Results Compared with control. striatal dopamine and its metabolites showed a marked reduction ( P ＜0. 01 ), and nigrostriatal TH expression also decreased remarkably ( P ＜ 0. 05 ). while BVRB expression was significantly upregulated in the nigrostriatal system of both models. Conclusion Nigrostriatal BVRB up-regulation might be related to PD oxidative stress.关键词
帕金森病模型/黑质纹状体系统/胆绿素还原酶B/1-甲基-4-苯基-1,2,3,6-四氢吡啶/6-羟基多巴/多巴胺/酪氨酸羟化酶Key words
Parkinson,s disease model/ nigrostriatal system/ BVRB/ MPTP/ 6-OHDA/ dopamine/ tyrosine hydroxylase分类
医药卫生引用本文复制引用
周冰莹,赵欣,孙泓,姜尧,蒲小平..帕金森病小鼠和大鼠模型黑质纹状体中胆绿素还原酶B的表达[J].中国药理学通报,2011,27(8):1126-1130,5.基金项目
国家自然科学基金资助项目(No 30973889) （No 30973889）
