中国药理学通报2011，Vol.27Issue(8)：1051-1054,4.DOI:10.3969/j.issn.1001-1978.2011.08.005
穿孔膜片钳方法记录L型钙通道及脱氢紫堇碱对其影响的研究
Recording L-type calcium channel current by perforated patch clamp and effect of dehydrocorydaline on L-type calcium channel
摘要
Abstract
Aim To observe the difference of recording L-type calcium channel currents over time through by whole cell patch clamp and perforated patch clamp method and the effect of dehydrocorydaline on L-type calcium channel by perforated patch clamp method.Methods Whole cell patch clamp and perforated patch clamp were used to record L-type calcium channel current in acutely isolated rat ventricular myocytes.Results The L-type calcium channel current peak recorded by whole cell patch clamp decayed of the ( 34 ±23 )％ ( n =10 ) within 15 minutes, while using perforated patch clamp, L-type calcium channel current peak attenuated ( 2. 7 ±3. 4 )％ ( n =9 ) within 15 minutes; The inhibitory effect of ginsenoside Re( 100μmol · L-1 ) could be clearlv recorded by perforated patch clamp method. while the current decaying produced by whole cell patch clamp almost completely overshadowed the effects of ginsenoside Re. Dehydrocorydaline ( 10, 100 μmol · L-1 ) could inhibit L-type calcium channel current peak and the inhibitory rates were ( 9 ±7. 5 )％( n =5 )and ( 28. 6 ±8. 5 )％( n =5 )individually. Conclusions Perforated patch clamp method has more stability and accuracy than ordinary whole-cell patch clamp technique in recording L-type calcium channel current; dehydrocorydaline can suppress L-type calcium channel in a concentration-dependent manner.关键词
穿孔膜片钳/全细胞膜片钳/心室肌细胞/L型钙通道/电流衰减/脱氢紫堇碱Key words
perforated patch clamp/ whole cell patch clamp / ventricular myocytes / L-type calcium channel current decaying / dehydrocorydaline分类
医药卫生引用本文复制引用
孟红旭,王宝,刘建勋..穿孔膜片钳方法记录L型钙通道及脱氢紫堇碱对其影响的研究[J].中国药理学通报,2011,27(8):1051-1054,4.基金项目
国家重大新药创新项目(No 2009ZX09301-005) （No 2009ZX09301-005）
国家自然科学基金重点项目(No 30830118) （No 30830118）
