中国药理学通报2011，Vol.27Issue(8)：1086-1090,5.DOI:10.3969/j.issn.1001-1978.2011.08.013
香椿果抗补体活性多酚对补体损伤神经细胞的保护作用研究
Effects of an anticomplementary polyphenol from the seed of Toona sinensis on complement-injured SH-SY5Y cells
摘要
Abstract
Aim To evaluate the protective effects of XCG-7. an anticomplementary polyphenol from the seed of Toona sinen.si.s( Meliaceae ) on complement-injured SH-SY5Y cells. Methods A model of neuronal injury was established on SH-SY5Y cells through activation of complement by cobra venom factor, an alternative pathway-specific activator. SH-SY5Y cells were exposed to different concentrations of XCG -7 . and then the release of lactate dehydrogenase and cell viability were measured at 24 h and 48 h. and the activity of caspase-3/7 was determined at 24 h. Results The anticomplementary polyphenol XCG-7 promoted SHSY5Y cell proliferation. The polyphenol inhibited the reduction of cell viability induced by activated complement , and moderated the release of lactate dehydrogenase . and decreased the apoptosis in the cells. Conclusion The anticomplementary polyphenol XCG-7 from the seed of Toona .smensis can promote SH-SY5Y cell growth, and protect SH-SY5Y cells from complementinduced injury.关键词
补体/SH-SY5Y细胞/多酚/香椿果/神经细胞损伤/细胞活力/乳酸脱氢酶/神经细胞凋亡/脑缺血Key words
complement/ SH-SY5Y cells/ polyphenol/ Toona .sinen.sis( Metiaceae )/ neuronal injury/ cell viability/ lactate dehydrogenase/ neuronal apoptosis/cerebral ischemia分类
医药卫生引用本文复制引用
赵琼,孙黔云,杨庆雄..香椿果抗补体活性多酚对补体损伤神经细胞的保护作用研究[J].中国药理学通报,2011,27(8):1086-1090,5.基金项目
国家重点基础研究发展计划(973计划)资助项目(No 2009CB526512,2007CB516813) （973计划）
贵州省优秀青年科技人才资助项目(No 2005-0510) （No 2005-0510）
