四川大学学报（自然科学版）2011，Vol.48Issue(3)：697-702,6.DOI:10.3969/j.issn.0490-6756.2011.03.040
甘蓝型油菜BnFAD8基因编码序列的克隆和表达谱分析
Cloning and expression characteristics of fatty acid desaturase 8 gene from rape (Brassica napus L.)
摘要
Abstract
Brassica napus fatty acid desaturase 8 (FAD8) belongs to cold-induced omega 3 fatty acid desaturase.By aligning Arabidopsis FAD8 and other homologous FAD8 genes, one highly conserved sequence of FAD8 from rape was cloned in study.Using the FAD8 conserved sequence as a querying probe, highly homologous EST were obtained from database of GenBank and a putative complete ORF of 1299 bp (named: BnFAD8) was assembled according to the rape clone.Furthermore, the full length cDNA of Brassica napus FAD8 was cloned by RT-PCR with two primers designed based on this assembled cDNA sequence.Features of plasmid-located o3 fatty acid desaturase were found in sequence analysis of cDNA cloned.As shown in expression profile result, BnFAD8 was highly expressed in leaves at 8 ℃, but only trace-level expression in leaves at 22 ℃, which suggested that BnFAD8 involved in the process of rape cold acclimation.关键词
甘蓝型油菜/基因克隆/BnFAD8/低温诱导表达Key words
Brassica napus/ gene cloning/ BnFAD8/ cold-induced gene分类
生物科学引用本文复制引用
刘绵学,王茂华,向俊蓓,杨毅,李旭锋..甘蓝型油菜BnFAD8基因编码序列的克隆和表达谱分析[J].四川大学学报（自然科学版）,2011,48(3):697-702,6.基金项目
国家自然科学基金(30471095) （30471095）
