山东医药2011，Vol.51Issue(19)：18-20,3.
人TAT-PDX-1融合蛋白在大肠杆菌中的表达与纯化
Expression and purification of human TAT-hPDX-1 fusion protein in E.coli
摘要
Abstract
Objective To construct a combinated vector containing TAT and human pancreatic duodenal homeobox-1 ( hPDX-1 ), and to purify its fusion protein. Methods The target gene hPDX-1 was obtained from human pancreatic tissue byRT-PCR, and then PDX-1 and TAT were cloned to expressive vector pET-28a by; fusion protein was induced and expressed by IPTG; fusion protein was purified by Ni-NTA agaros, and detected by western blot. Results PDX-1 was effectively amplified and the TAT-PDX-1 gene sequencing showed sequence as scheduled; the fusion protein was successfully expressed in E. coli and purified. Conclusions Expression product of TAT-hPDX-1 fusion gene can be obtained successfully, and the results may lay a good basis for manufacture and clinical application of the TAT-hPDX-1.关键词
原核表达/蛋白转导域/胰十二指肠同源盒基因-1Key words
prokaryotic expression/ protein transduction domain/ pancreatic duodenal homeobox-1分类
医药卫生引用本文复制引用
苗成,宋波,王茜,张宁,韩志强,许予明..人TAT-PDX-1融合蛋白在大肠杆菌中的表达与纯化[J].山东医药,2011,51(19):18-20,3.基金项目
河南省杰出青年科学基金资助项目(074100510001). （074100510001）
