中国药理学通报2011，Vol.27Issue(8)：1131-1135,5.DOI:10.3969/j.issn.1001-1978.2011.08.023
大鼠伏核内注射吗啡及其受体阻断剂对其Nogo-A和CGRP mRNA表达的影响
Effects of morphine and its receptor blocking agents on the expression of Nogo-A and CGRP mRNA in nucleus accumbens of rats
摘要
Abstract
Aim The present study was performed to investigate the role of Nogo-A in pain modulation in the nucleus accumbens ( NAc ) of rats. Methods The inflammatory pain models in rats were prepared in the present study. In different groups,each rat was respectively injected saline , morphine and naloxone to the NAc for 1 μl. After 60 min, the brain tissues in the NAc were obtained. The real time quantitative RT-PCR technique was used for Nogo-A and CGRP mRNA recording and detected the changes of Nogo-A and CGRP mRNA with β-actin as an endogenous control. CT value of Nogo-A and CGRP mRNA in the NAc of rats was recorded and the relative content of every sample against its endogenous control was calculated. The influence of inflammatory pain, morphine or naloxone on Nogo-A and CGRP mRNA expression in the NAc was analyzed. Results Compared with blank control group,the expression of Nogo-A mRNA decreased significantly( P ＜0. 05 ) and CCRP mRNA increased significantly( P ＜ 0. 01 ) in hyperalgesia model control group. Compared with hyperalgesia model control group ,the expression of Nogo-A mRNA increased significantly( P ＜ 0. 01 ) and CCRP mRNA decreased significantly( P ＜ 0. 05 )in morphine group; and the expression of Nogo-A mRNA decreased significantly( P ＜0. 05 ) and CGRP mRNA increased significantly( P ＜0. 05 )in naloxone group. Conclusion When the rats are injected morphine or naloxone to the NAc, the expressions of Nogo-A and CGRP mRNA are both influenced. And the relationship between Nogo-A and CGRP is the negative regulation.关键词
吗啡/伏核/Nogo-A/降钙素基因相关肽/痛觉调制/实时定量逆转录聚合酶链式反应/大鼠Key words
morphine/ nucleus accumbens/ Nogo-A/CGRP/ pain modulation/ RT-PCR/ rat分类
医药卫生引用本文复制引用
王燕,孟蒂,李宁..大鼠伏核内注射吗啡及其受体阻断剂对其Nogo-A和CGRP mRNA表达的影响[J].中国药理学通报,2011,27(8):1131-1135,5.基金项目
山东省自然科学基金资助项目(No 2009ZRB019FO) （No 2009ZRB019FO）
