中国药理学通报2011，Vol.27Issue(8)：1135-1138,4.DOI:10.3969/j.issn.1001-1978.2011.08.024
外源性多胺对老年大鼠抗衰老作用的实验研究
Experimental study on anti-aging action of exogenous polyamines in rats
摘要
Abstract
Aim To observe the anti-aging effects and possible mechanism of exogenous polyamines in oldaged rats. Methods Polyamines ( spermidine and spermine ) were given through intragastric administration to 18 months old-aged wistar rats as polyamines administration group ( polyamines ), while wistar rats of the same months acted as old control group ( old ),wistar rats of 3 months as young control group ( young ). Biochemical tests were conducted of the levels of malondialdehyde ( MDA ) and the activity of superoxide dismutase ( SOD ) in these rats' heart and liver tissues. The levels of TNF-α and IL-1β were determined by Enzyme-linked immunosorbent assay. Myocardial ultrastructures were observed with transmission electron microscope. Results In comparison with the young group, in the old group, the activity of SOD of heart and liver tissues decreased significantly, the contents of MDA as well as TNF-α and IL-1β increased dramatically. Compared with the old group, in the polyamines group, the activity of SOD increased strikingly while the levels of MDA. TNF-α and IL-1β decreased remarkably. From the transmission electron microscope, myocardia in the old control group was found to have lots of lipofuscin particle deposition; mitochondria increased in size, the number decreased.and the mitochondrial cristae presented flocculent changes. In the polyamines group, myocardial sarcomere structures showed clarity; mitochondrial matrix showed more density and mitochondrial cristae arranged in neat rows. and lipofuscin granules were seen occasionally. Conclusion To some extent. exogenous polyamines can delay the natural aging process of rats .and the action mechanism may be associated with improving the antioxidant capacity of old rats ' heart and liver, and inhibiting the generation of pro-inflammatory mediators.关键词
多胺/衰老/肝脏/心脏/大鼠/自由基/炎症Key words
polyamines/ aging/ liver/ heart/ rat/ free radical/ inflammation分类
医药卫生引用本文复制引用
薛过,王伟伟,赵雅君,张伟华,王丽娜,李红霞,张力,徐长庆..外源性多胺对老年大鼠抗衰老作用的实验研究[J].中国药理学通报,2011,27(8):1135-1138,4.基金项目
黑龙江省博士后启动基金(No LRB05-266) （No LRB05-266）
