中国药理学通报2011，Vol.27Issue(8)：1156-1160,5.DOI:10.3969/j.issn.1001-1978.2011.08.029
吡那地尔后处理在减轻家兔心肌缺血/再灌注损伤中的作用
Effects of Pinacidil postconditioning on relief of myocardial reperfusion injury
摘要
Abstract
Aim This study aimed to investigate effects of Pinacidil postconditioning on the relief of myocardial reperfusion injury in rabbit in vivo and explore its possible mechanisms. Methods Forty healthy adult male rabbits were randomly divided into five groups ( n = 8 in each group ): Group1 ( Sham ), Group2 ( ischemic reperfusion ), Group3 ( ischemic postconditioning ),Group 4( Pinacidil postconditioning ), Group5 ( Pinacidil and 5-HD postconditioning ). The model of ischemic reperfusion injury was made by occluding the left anterior descending artery for 30 min and reperfusing for 120 min in rabbit heart. The index of cardiac function, plasma creatine kinase activity and malondialdehyde content were measured and compared at baseline,the end of ischemia, and after 120 min of reperfusion respectively. Myocardial ischemic,infarct size, expression of Bcl-2 and Bax mRNA in myocardium were determined at the end of the experiment. Result At 120 min of reperfusion. compared with Group 2 and Group 5 , LVSP was significantly higher in Group 3 and 4 ( P ＜ 0. 05 ); LVEDP, plasma CK activity, and Plasma MDA content were significantly lower ( P ＞ 0. 05 );myocardial infarct size was significantly reduced in Group 3 and 4 ( P ＜ 0. 05 ) ; expression of Bcl-2 mRNA increased remarkably ; Bax decreased remarkably ( P ＜0. 05 ). Conclusion Pinacidil postconditioning provides potent reduction of reperfusion injury, which is similar to the cardioprotective effect of ischemic postconditioning. The potential mechanisms of this phenomenon might be associated with mitochondrial ATPsensitive potassium channel opening, increased expression of Bcl-2 mRNA and decreased Bax mRNA.关键词
吡那地尔/缺血后处理/再灌注损伤/心肌保护/ATP敏感性钾通道/Bcl-2家族Key words
Pinacidil / ischemicpostconditioning / reperfusion injury / cardioprotection / ATP-sensitive potassium channel / Bcl-2 family分类
医药卫生引用本文复制引用
田涛,关旭东,马宾,王明霞,蔡鑫,刘进军,方羚,吴士礼,高琴,于影..吡那地尔后处理在减轻家兔心肌缺血/再灌注损伤中的作用[J].中国药理学通报,2011,27(8):1156-1160,5.基金项目
安徽省卫生厅医学科研资助项目(No 2010C079) （No 2010C079）
