中国组织工程研究与临床康复2011，Vol.15Issue(20)：3711-3714,4.DOI:10.3969/j.issn.1673-8225.2011.20.024
肝星状细胞增殖与p38丝裂原活化蛋白激酶信号传导通路的关系
Correlation between acetaldehyde-induced proliferation of hepatic stellate cells and p38 mitogen-activated protein kinase signal transduction pathway
郑人源 1蒋明德 2梅浙川 1卓强 1叶平 2唐文2
作者信息
- 1. 重庆医科大学附属第二医院消化内科,重庆市,400016
- 2. 解放军成都军区总医院消化内科,四川省成都市610083
- 折叠
摘要
Abstract
BACKGROUND: Activation and proliferation of hepatic stellate cells (HSCs) leads to hepatic fibrosis, and p38 mitogen-activated protein kinase (p38MAPK) signaling pathway has a role in regulating cell proliferation.OBJECTIVE: To explore the p38MAPK activity and cell proliferation of acetaldehyde-induced rat HSCs treated with SB203580. METHODS: Rat HSC strains were cultured in vitro, and divided into blank group, acetaldehyde control group and SB203580 group. The proliferation of HSCs was evaluated by MTT colorimetric assay and the variability of phosphorylated-p38 was examined by Western blot.RESULTS AND CONCLUSION: p38 activity increased in acetaldehyde-induced HSCs, and HSCs proliferated significantly; SB203580 (5, 10, 20 μmol/L) could block the activity of p38 in the cells, and inhibit acetaldehyde-induced HSCs proliferation (P ＜ 0.05), when the concentration was increased to 30 μmol/L, its inhibitive effect on HSCs proliferation was more significant, and the expression of p-p38 decreased relatively (P ＜ 0.05). The results showed that inhibition of p38MAPK activity can affect HSCs proliferation; p38 signaling pathway may play an important role in the regulation of HSCs proliferation.关键词
肝纤维化/肝星状细胞/p38/SB203580/细胞增殖/组织构建分类
医药卫生引用本文复制引用
郑人源,蒋明德,梅浙川,卓强,叶平,唐文..肝星状细胞增殖与p38丝裂原活化蛋白激酶信号传导通路的关系[J].中国组织工程研究与临床康复,2011,15(20):3711-3714,4.
