广东医学2011，Vol.32Issue(14)：1789-1792,4.
锂对脆性X综合征小鼠模型的跳台行为及糖原合成酶激酶3β活性的影响
The effects of lithium on memory ability and glycogen synthase kinase-3β activity in mouse model of fragile X syndrome
摘要
Abstract
Objective To evaluate whether lithium ameliorates the learning and memory behavior, and glycogen synthase kinase -3β ( GSK3β ) activity in FVB Fmr1 gene knockout ( ko ) mice. Methods The 30 - day - old Fmr1 gene ko mice. and wild - type mice ( wt mice ) were included. Normal saline treatment and lithium treatment of 30, 60 ,90 , 120 and 200 mg/kg were carried out on both ko and wt mice, respectively. Behavioral latency and error frequencies in step - down passive avoidance test were recorded after treatment. Meanwhile , the expression of GSK3β and phosphorylated GSK3β ( P - GSK3β ) in hippocampus and cortex were quantified with Western - blot. Results In mice without treatment, shorter behavioral latency, and higher error in step - down test were revealed in ko mice, when comparing with that of wt mice. Meanwhile, lower expression of P - GSK3β was observed in ko mice than that in wt mice. These differences were ameliorated by lithium treatment, with maximum efficacy in dose of 30 and 60 mg/kg. Conclusion Lithium improves learning and memory ability in ko mice, via up - regulation of P - GSK3β, providing application in treatment of Fmr1 gene ko mice.关键词
脆性X综合征/跳台实验/学习记忆/糖原合成酶激酶3β/氯化锂Key words
fragile X Syndrome/ step - down passive avoidance test/ learning and memory/ glycogen synthase kinase3β / lithium chloride引用本文复制引用
杨泉,陈盛强,曹开谊,张伟雯,黄越玲,刘国彬,沈岩松,孙卫文,李敏雄,戴丽军..锂对脆性X综合征小鼠模型的跳台行为及糖原合成酶激酶3β活性的影响[J].广东医学,2011,32(14):1789-1792,4.基金项目
国家自然科学基金资助项目(编号:30870876),广东省自然科学基金资助项目(编号:815101700100005),广东省科技计划项目(编号:2008B030301371),广州市医药卫生科技一般引导项目(编号:2009-YB-167) （编号:30870876）
