海洋科学2011，Vol.35Issue(3)：17-22,49,7.
乌苏里拟鲿野生群体和人工养殖群体遗传多样性的比较研究
Genetic diversity analyses of wild and cultured Pseudobagrus ussuriensis populations
摘要
Abstract
The sequence-related amplified polymorphism (SRAP) molecular marker technique was used to compare genetic structures of three populations (one wild and two cultured) of Pseudobagrus ussuriensis.Samples of wild population were collected from Jiangsu Hongze Lake and the two cultured populations from Fisheries Research Institute of Huaian (F2 generation) and Suzhou Dongshan Aquatic Breeding Plants (F3 generation).Twelve pairs of SRAP primers were selected from 100 primer combinations.Two hundred and two amplified loci were obtained from the three populations, among which 130 were polymorphic.The percentage of polyrnorphic loci in the Hongze Lake (HL) population, Huaian populations (HA), Suzhou Dongshan populations (SZ) was 63.13％, 56.54％ or 54.88％, respectively.The results indicated that genetic polyrnorphism decreased in two cultured populations.The Nei's gene diversity of three populations was 0.2641, 0.2546 or 0.2469, respectively; the Shannon's Information index of three populations was 0.4118, 0.4050 or 0.3861, respectively.The genetic distance between wild population and two cultured ones were 0.1730 and 0.1104, while the genetic distance between two cultured ones was 0.1087.The noticeable decrease in the number of rare loci and the increase in the number of homozygous recessive loci in the cultured population suggested a considerable loss of low frequency alleles in the cultured populations,which might have resulted from small effective population sizes during artificial seed production.In the future P.ussuriensis artificial propagation should be chosen to large sufficiently and representative parent groups to maintain the good traits to retain maximum genetic diversity.关键词
乌苏里拟鲿(Pseudobagrus ussuriensis)/野生群体/人工养殖群体/遗传多样性/SRAPKey words
Pseudobagrus ussuriensis/ wild population/ cultured population/ genetic diversity/ SRAP分类
农业科技引用本文复制引用
徐汗福,黄鹤忠,范皖苏,何华敏,贾一何..乌苏里拟鲿野生群体和人工养殖群体遗传多样性的比较研究[J].海洋科学,2011,35(3):17-22,49,7.基金项目
江苏省科技厅科技支撑项目(BE2009340) （BE2009340）
苏州市科技局科技支撑项目(SNG0912) （SNG0912）
