中国药房2011，Vol.22Issue(22)：2056-2058,3.
HPLC-MS/MS法测定人血浆中氯法拉滨的浓度
Determination of the Concentration of Clofarabine in Human Plasma by HPLC-MS/MS
朱宝英 1赵立波 2王茜 2张春燕 2陈欣 2黄静 2方翼1
作者信息
- 1. 贵阳医学院,贵阳市,550004
- 2. 北京大学人民医院,北京市,100044
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the concentration determination of clofarabine in human plasma. METHODS: The plasma samples were extracted with methyl tert-butyl ether, then was determined by HPLC-MS/MS. Thermo C18 column was used as analytic column with mobile phase consisted of acetonitrile-water (250∶3, containing 4 rmmol· L-1 ammonium acetate and 0.3％ formic acid) at flow rate of 0.5 mL·min-1. The injection volume was 10 μL and cladribine was used as the internal standard (IS). Clofarabine and cladribine were detected on multiple reaction monitoring (MRM) by the transitions from the precursor to the product ion (m/z 304.2→170.0 and m/z 286.1→170.0). And the total running time was 5 min. RESULTS: The retention time of clofarabine and cladribine were 3.83 min and 3.90 min. The linear range of clofarabine was 2.5～500 ng·mL-1(r=0.999 9). The RSD of intra-day and inter-day were both less than 14％ and the average recovery was 95.64％ ～99.23％. CONCLUSION: Established method is simple, sensitive. rapid and accurate, and it is suitable for plasma concentration determination and pharmacokinetics study of clofarabine.关键词
氯法拉滨/高效液相色谱串联质谱电喷雾法/血药浓度/药动学Key words
Clofarabine/ HPLC-MS/MS/ Plasma concentration/ Pharmacokinetics分类
医药卫生引用本文复制引用
朱宝英,赵立波,王茜,张春燕,陈欣,黄静,方翼..HPLC-MS/MS法测定人血浆中氯法拉滨的浓度[J].中国药房,2011,22(22):2056-2058,3.
