中国药房2011，Vol.22Issue(23)：2167-2168,2.
RP-HPLC法测定不同采收期广东紫珠、大叶紫珠中木犀草素的含量
Content Determination of Luteolin in Callicarpa kwangtungensis and Callicarpa macrophylla in Different Harvesting Time by RP-HPLC
摘要
Abstract
OBJECTIVE: To determine the content of luteolin in Callicarpa kwangtungensis and Callicarpa macrophylla in different harvesting time and to explore the rules of dynamic change of luteolin content. METHODS: RP-HPLC was performed on Alltech C18(250 mm×4.6 mm, 5 μm) column with column temperature at 30 ℃. The mobile phase consisted of methanol-0.3 ％ phosphate aqueous solution (47: 53) at flow rate of 1 mL·min-1, and the detection wavelength was set at 352 nm. RESULTS: The linear range of luteolin was 0.015～0.300 μg (r=0.999 9) with an average recovery of 98.52％ (RSD= 1.84％ ,n=6). The content of luteolin increased gradually with the growth of plants and reached the peak at the end of growth period. The content of luteolin in C.macrophylla was commonly higher than in C. kuangtungensi. CONCLUSION: Results of study can provide scientific basis for natural cultivation or artificial cultivation of C. kwangtungensi and C. macrophylla.关键词
广东紫珠/大叶紫珠/木犀草素/含量测定/反相高效液相色谱法Key words
Callicarpa kwangtungensi/ Callicarpa macrophylla/ Luteolin/ Content determination/ RP-HPLC分类
医药卫生引用本文复制引用
周日宝,刘笑蓉,唐丽君,刘佳胜..RP-HPLC法测定不同采收期广东紫珠、大叶紫珠中木犀草素的含量[J].中国药房,2011,22(23):2167-2168,2.基金项目
湖南省高校创新平台开放基金项目(09K061) （09K061）
湖南省中医药科研计划项目(2009010) （2009010）
