孙莲 1阿合买提江 1马晓丽 1古力包斯坦·巴拉提1
作者信息
- 1. 新疆医科大学药学院化学教研室,乌鲁木齐市,830011
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of rutin, isoquercitrin, quercetin, chlorogenic acid in Morus ningra.METHODS: High performance capillary electrophoresis (HPCE) method was adopted with 50 mmol·L-1 borax solution (pH=9.6) as buffer solution under voltage of 20 kV and temperature of 25 ℃.The detection wavelength was set at 254 nm.RESULTS: The linear ranges of rutin, isoquercitrin, chlorogenic acid, quercetin were 0.18～105 μg·mL-1, 0.15～100 μg·mL-1, 2.65～169 μg·mL-1 and 0.11～79 μg· mL-1 (r=0.999 2,0.998 9, 0.999 2,0.999 7), respectively.Average recoveries were 98.4％, 97.6％, 97.3％, 100.3％, respectively(RSD=1.1％ ,3.6％ ,2.1％ ,2.6％ ,n=9).CONCLUSION: The method is simple,rapid, reproducibility, accurate and reliable.It can be used for the content determination and quality control of 4 kinds of total flavonoids in M.nigra.关键词
高效毛细管电泳法/黑桑/芦丁/槲皮素/异槲皮苷/绿原酸/含量测定Key words
High performance capillary electrophoresis/ Morus nigra/ Rutin/ Quercetin/ Isoquercitrin/ Chlorogenic acid/Content determination分类
医药卫生
