中国医科大学学报2011，Vol.40Issue(5)：397-399,411,4.DOI:CNKI:21-1227/R.20110523.1816.027
血红素氧合酶在脑膜炎中的差异性表达及SnPP的保护作用
The Differiential Expression of Hemoxygenases and the Protection of SnPP in Experimental Meningitis
摘要
Abstract
Objective To study the relationship between heme degrading enzyme hemoxygenase activity and free iron increase during brain meningitis, and to investigate the protective effect of Sn-protoporphyrin (SnPP) in the inhibition of free iron release.Methods Wistar pups were used for meningitis brain models.Heme oxygenase (HO) -1 and -2 expression was measured by Western blot quantitatively and immunohistochemistry.SnPP was injected subcutaneously and free iron was measured with Ferene-S method.Bilirubin was detected by immunohistochemical method to examine hemoxygenase activities.Results Compared with control group,HO-1 level in the infected brain was significantly increased along with the free iron increase.HO-1 expression colocalized with free iron in neurons and glial cells.HO-2 level remained low and unchanged.SnPP significantly reduced the free iron release and bilirubin formation.Conclusion Pneumococcal meningitis was associated with an increase of free iron in the brain which was originated from HO-1 activity increase but not HO-2,SnPP was able to prevent the free iron increase and supposed to be brain protective to oxidative damage.关键词
大鼠/游离铁/血红素氧合酶/锡原卟啉Key words
rat/ free iron/ hemoxygenase/ SnPP分类
医药卫生引用本文复制引用
任昊,翟效月..血红素氧合酶在脑膜炎中的差异性表达及SnPP的保护作用[J].中国医科大学学报,2011,40(5):397-399,411,4.基金项目
高等学校博士学科点专项科研基金资助项目(200801590016) （200801590016）
