海洋科学2011，Vol.35Issue(4)：21-25,5.
裙带菜配子体总RNA提取的比较研究
Comparison of methods for total RNA extraction from Undaria pinnatifida gametophyte
摘要
Abstract
In this paper, modified SDS method, CTAB method, and Trizol and RNAiso Reagent kits were applied to extract total RNA from Undaria pinnatifida gametophyte.We compared the quality and purity of the extracted RNAs and used RT-PCR to examine whether the RNAs were functionally active.The results showed that the Trizol and RNAiso Reagent kits were not suitable to seaweed RNA isolation, because there was no clear band detected on the gel.The RNA isolated by the CTAB method showed smear bands and also was contaminated by genomic DNA.However, the modified SDS method could give birth to good quality RNAs, although there was a little genomic DNA contamination.The OD260/OD280 ratio of the RNA was between 1.8 and 2.0.These RNAs could be used for RT-PCR.So the modified SDS method is a very good method for extracting total RNAs from gametophyte of U.pinnatifida and some other seaweeds.关键词
裙带菜(Undaria pinnatifida)/配子体/RNA提取/RT-PCRKey words
Undaria pinnatifida/ gametophyte/ RNA extraction/ RT-PCR分类
农业科技引用本文复制引用
李世国,佟少明,侯和胜..裙带菜配子体总RNA提取的比较研究[J].海洋科学,2011,35(4):21-25,5.基金项目
中国科学院实验海洋生物学重点实验室开放基金项目 （）
