中国医科大学学报2011，Vol.40Issue(5)：408-411,4.DOI:CNKI:21-1227/R.20110523.1816.030
透明质酸钠对大鼠成肌细胞体外成骨分化的影响
Effect of Sodium Hyaluronate on In Vitro Osteoplasfic Differentiation of Rat's Myoblasts
张玉强 1李游 2王岩峰 1王伟 3吕刚4
作者信息
- 1. 中国医科大学附属第一医院骨科,沈阳,110001
- 2. 中国医科大学附属盛京医院眼科,沈阳,110004
- 3. 锦州市中心医院骨科,辽宁锦州,121001
- 4. 辽宁医学院附属第一医院骨科,辽宁锦州,121001
- 折叠
摘要
Abstract
Objective To investigate the effects of sodium hyaluronate solution on the osteoplastic diferentation of rat's myoblasts. Methods The minced muscles were digested with mixed digestive juices,followed by the isolation,culture and purification of the myoblasts. The third subculture myoblasts were cultured in two growth media,in which experimental group containing 0.1％ sodium hyaluronate and 500 ng/mL rhBMP-2, the control group containing 500 ng/mL rhBMP-2, respectively. The morphological change of induced myoblasts in each medium was observed carefully. Three weeks later,a series of examination were performed,including:ALP activity,type Ⅰ collagen immunohistochemistry, and new calcifying nodules staining with Alizarin red S and ALP. Results The morphological change in each medium was similar during the induced process. There was a difference between the two groups on ALP activity,but the ALP activity increased and there was no statistic difference (P ＞ 0.05 ). The immunohistochemistry of type Ⅰ collagen was positive,a great quantity of new calcifying nodules was seen by the staining of Alizarin red S and ALP. Conclusion Sodium hyaluronate could not inhibit the osteoplastic differentiation of rat's myoblasts in vitro,it could be a proper media for myoblasts.关键词
透明质酸钠/成肌细胞/骨形态发生蛋白Key words
sodium hyaluronate/ myoblasts/ bone morphogenetic protein分类
医药卫生引用本文复制引用
张玉强,李游,王岩峰,王伟,吕刚..透明质酸钠对大鼠成肌细胞体外成骨分化的影响[J].中国医科大学学报,2011,40(5):408-411,4.
