海洋科学2011，Vol.35Issue(4)：70-76,7.
坛紫菜磷酸烯醇式丙酮酸羧化酶基因的克隆与序列分析
Cloning and analysis of the phosphoenolpyruvate carboxylase gene of Porphyra haitanesis (Rhodophyta)
摘要
Abstract
Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) plays an important role in photosynthesis.To get a deeper insight into the PEPC gene of Porphyra haitanensis T.J.Chang et B.F.Zheng (Bangiales, Rhodophyta),we cloned a partial-length cDNA by homology cloning and RACE.This sequence includes 2538bp encoding a propetide of 846 amino acid residues.The deduced polypeptide shows high identities with the PEPC genes ranging from bacteria and unicellular algae to green plant.Phylogenetic tree analysis shows that he PEPC gene from the Porphyra haitanensis is a C3- like PEPC and more closely related to the ancestral type.关键词
坛紫菜/PEPC基因/序列分析/系统进化分析Key words
Porphyra haitanensis/ phosphoenolpyruvate carboxylase gene/ sequence analysis/ phylogenetic tree analysis分类
生物科学引用本文复制引用
张晓娟,王广策,何林文,陈昌生..坛紫菜磷酸烯醇式丙酮酸羧化酶基因的克隆与序列分析[J].海洋科学,2011,35(4):70-76,7.基金项目
国家自然科学基金重点项目(30830015) （30830015）
海洋公益性行业科研专项项目(201105008-2,201105023-7) （201105008-2,201105023-7）
