中国实用儿科杂志2011，Vol.26Issue(6)：439-442,4.
原发性肾病综合征患儿外周血Zeta链改变临床意义分析
Significant change of Zeta chain of peripheral blood in children with primary nephritic syndrome
郑荣浩 1朱光华 2王平 3刘家应 2夏敏 2何威逊3
作者信息
- 1. 湖北省妇幼保健院(湖北省妇女儿童医院)儿内科
- 2. 上海交通大学附属儿童医院,检验科,上海,200040
- 3. 上海交通大学附属儿童医院,肾脏科,上海,200040
- 折叠
摘要
Abstract
Objective To detect the expression level of Zeta chain, and to probe its influence on the immunodeficiency and its role in children with PNS. Methods Twenty-six children in first-onset, twenty-two children in relapse and thirty children in remission with PNS were studied, respectively.Twenty-one children were also studied as healthy control.The expression level of Zeta chain was examined by three-color flow-cytometry. Results The mean fluorescence intensity (MFI) of Zeta chain in T and NK cell of peripheral blood was significantly reduced in first-onset and relapse as compared with healthy controls, respectively. (P ＜ 0.05), while an significant increase was observed during remission as compared with first-onset and relapse, respectively. (P ＜ 0.05). However, the proportion of T and NK cell of peripheral blood between groups was not significantly different (P ＞ 0.05). Between remission and healthy controls, the MFI of Zeta chain in T and NK cell of peripheral blood was not significantly difference (P ＞ 0.05). The MFI of Zeta chain in T and NK cell of peripheral blood was also not significantly difference between first-onset and relapse (P ＞ 0.05). Among these children with PNS, when nine children in first-onset and five children in relapse achieved remission, the expression level of Zeta chain was increased significantly (P ＜ 0.05), and there were two children in first-onset whose Zeta chain expression level was reduced again when relapse. Conclusions The expression level of Zeta chain is significantly reduced in first-onset and relapse, and it is similar to that expressd in normal control in remission in children with PNS. Therefore, Zeta chain might be involved in the process of first-onset and relapse. The Zeta chain of peripheral blood might be one of markers which might be helpful to monitor disease condition and evaluate the effect of treatment in children with PNS关键词
Zeta链/肾病综合征/T细胞/自然杀伤细胞/流式细胞术Key words
Zeta chain/ nephrotic syndrome/ T cell/ NK cell/ flow-cytometry分类
医药卫生引用本文复制引用
郑荣浩,朱光华,王平,刘家应,夏敏,何威逊..原发性肾病综合征患儿外周血Zeta链改变临床意义分析[J].中国实用儿科杂志,2011,26(6):439-442,4.
