中国组织工程研究与临床康复2011，Vol.15Issue(20)：3641-3644,4.DOI:10.3969/j.issn.1673-8225.2011.20.008
组织工程表皮与单核白血病细胞共培养的致敏性检测模型
A co-culture model of tissue engineering epidermis and THP-1 cells for sensitization testing
摘要
Abstract
BACKGROUND: Several studies have shown that tissue engineering skin are used for skin irritation testing. OBJECTIVE: To construct a co-culture model of tissue engineering epidermis and THP-1 cells, and to identify the specificity of the co-culture model to sensitizers.METHODS: Tissue engineering epidermis was constructed by human keratinocytes. Then, THP-1 cells were co-cultured tissue engineering epidermis. After 24 hours treatment on tissue engineering epidermis, the cultured medium and THP-1 cells were collected, respectively. ELISA was used to detect the concentration of interleukin 1beta (IL-1β) in the culture medium, and the mean fluorescence intensities (MFI) measured by flow cytometry were used to evaluate the expression of CD86 and CD54 on THP-1 cells.RESULTS AND CONCLUSION: After 14 days keratinocytes were cultured at air-liquid interface, HE staining showed 8-10 layers in tissue engineering epidermis. And pan-keratin immuohistochemistry staining was positive. After 24 hours treatment of chemicals, the concentration of IL-1β and MFIs of CD86 and CD54 in DNFB-treated group were higher that that in vehicle control group (P ＜ 0.05). The results prove that a co-culture model of tissue engineering epidermis and THP-1 cells was successfully established, and it can be used for sensitization test, which gives a new method for toxicity testing in vitro.关键词
组织工程表皮/单核白血病细胞/致敏性/替代毒理/皮肤组织构建分类
医药卫生引用本文复制引用
曹玉萍,周武庆,马鹏程,刘维达,李玲珺 ,陶玥 ,张孟丽,陶蕾,魏峻..组织工程表皮与单核白血病细胞共培养的致敏性检测模型[J].中国组织工程研究与临床康复,2011,15(20):3641-3644,4.基金项目
课题受江苏省自然科学基金项目(BK2008092)资助. （BK2008092）
