中国组织工程研究与临床康复2011，Vol.15Issue(20)：3645-3648,4.DOI:10.3969/j.issn.1673-8225.2011.20.009
兔骨内高压型膝关节骨性关节炎模型的建立
Construction of a high intraosseous pressure type rabbit osteoarthritis model
摘要
Abstract
BACKGROUND: Plenty of animal models of knee osteoarthritis has been established and used, most of them may not be right for the experimental study of Chinese medicine manipulation.OBJECTIVE: To determine the outcomes of constructed animal models of high intraosseous pressure knee osteoarthritis. METHODS: The inferior gluteal vein, femoral vein and great saphenous vein on the right side were exposed under aseptic conditions, ligatured and cutting off the veins. The animal with exposed vessel and normal animals were served as controls. All animals were sacrificed at 8 weeks after modeling. The structure changes of cartilage tissues were observed by gross observation, haematoxylin-eosin staining, and light microscope.RESULTS AND CONCLUSION: Articular surface of the model group was rough and dark-black, with obvious defects and osteophytosis, with the early and medial stages of osteoarthritis. Articular surface of sham-surgery group was rough, opacity and grey, with the early stages of osteoarthritis. Articular surface of the normal group was smooth, marginal regular, translucent shine for articular cartilage, with no obvious cartilage degeneration. The results revealed that, at 8 weeks after ligaturing the inferior gluteal vein, femoral vein and great saphenous vein would cause the early and medial stages of osteoarthritis in rabbits.关键词
膝关节/骨性关节炎/骨内高压/兔/动物实验分类
基础医学引用本文复制引用
戴七一,覃杰,吴兆沛,王雄,覃学流,袁经阳,林光琪,刘靖,阮萍,滕居赞,容向宾,韩杰..兔骨内高压型膝关节骨性关节炎模型的建立[J].中国组织工程研究与临床康复,2011,15(20):3645-3648,4.基金项目
国家自然科学基金项目(30860359)、广西区科技厅自然科学基金项目(桂科自0832178),课题名称:揉髌手法对兔膝关节软骨细胞凋亡及相关基因表达影响的实验研究. （30860359）
