中国组织工程研究与临床康复2011，Vol.15Issue(20)：3657-3661,5.DOI:10.3969/j.issn.1673-8225.2011.20.012
脊髓损伤模型大鼠神经胶质细胞增殖与胶质纤维酸性蛋白的表达
Proliferation of glial cells and expression of glial fibrillary acidic protein after spinal cord injury in rats
李宽新 1李锋2
作者信息
- 1. 华中科技大学同济医学院附属同济医院骨科,湖北省武汉市430022
- 2. 石河子大学医学院第一附属医院骨一科,新疆维吾尔自治区石河子市,832000
- 折叠
摘要
Abstract
BACKGROUND: Astrocytes can release various neurotrophic factors by cell lysis to promote spinal cord repair. OBJECTIVE: To observe the recovery of hjndlimb function and the expression of glial fibrillary acidic protein (GFAP) after spinal cord injury.METHODS: Spinal cord injury models at T9-10 were established with Allen’s method. After modeling, bone morphogenetic protein 7 was injected into the subarachnoid space. Normal SD rats with His protein injected into the subarachnoid space were used as controls. The behavioral evaluation was performed with the Basso, Beattie & Bresnahanlocomotor rating scale (BBB scale). GFAP expression was detected using hematoxylin-eosin staining and immunohistochemistry staining. RESULTS AND CONCLUSION: Based on BBB score, the hindlimb function of rats in the model group recovered 68％. GFAP expression in the model group increased gradually at 3 and 7 days after injury (P ＜ 0.05), and then decreased similar to the control group at 28 days after injury (P ＞ 0.05). The data indicated that injection of bone morphogenetic protein 7 into the subarachnoid space can induce the proliferation of glial cells, increase GFAP expression and then to promote the recovery of hindlimb function after spinal cord injury in rats.关键词
胶质纤维酸性蛋白/脊髓损伤/骨形态发生蛋白7/BBB评分/免疫组织化学/组织构建分类
医药卫生引用本文复制引用
李宽新,李锋..脊髓损伤模型大鼠神经胶质细胞增殖与胶质纤维酸性蛋白的表达[J].中国组织工程研究与临床康复,2011,15(20):3657-3661,5.
