昆明医学院学报2011，Vol.32Issue(5)：40-46,7.
低氧诱导对人肝癌SMMC7721细胞生物学行为的影响
Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro
摘要
Abstract
Objective To investigate the effect of hypoxia on human hepatoma carcmoma cell line SMMC7721 cultured in vitro. and to explore the possible molecular mechanism. Methods The chemical method using COCl2 to induce hypoxia environment of SMMC7721 cell was performed. The effect of hypoxia on cell growth, cycle and apoptosis. and adhesion and migration ability were detected by MTT assay, flow cytometry,cell adhesion test. and transwell chamber methods, respectively. RT-PCR and immunocytochemistry methods were used to detect HIF-1α , VEGF. MMP2 mRNA and protein expression. Results MTT assay showed that cell growth was inhibited under hypoxia induced by COCl2. Flow cytometry showed that cell apoptosis, necrosis rate were increased, CJ0/G1 cell cycle phase, S phase cells decreased, G2/M phase was increased flow cytometry showed that cell apoptosis, apoptosis and necrosis rate were increased, G0/G1 phase and S phase cells decreased,G2/M phase cells were increased 24 h after hypoxia. Adhesion test showed cell adhesion was enhanced 24 h after hypoxia ( P＜ 0.01), transwell chamber experiruents showed that the cell migration significantly increased 24 h after hypoxia compared with normoxia ( P ＜ 0.05). RT-PCR and immunocytochemical staining showed that mRNA and protein expression of HIF-1α , VEGF, MMP2 increased under hypoxic conditions compared with normoxia (P＜ 0.05 ). Conclusion The proliferation of SMMC7721 cell can be inhibited by hypoxia, resulting in the restriction of SMMC7721 cell in its G2/M phase of the cell cycle and enhance apoptosis and necrosis.HIF-1α and its downstream target genes VEGF and MMP2 mRNA and protein expression are involved in the biological behavior changes.关键词
肝癌细胞/缺氧/细胞周期/凋亡/粘附/迁移/缺氧诱导因子1-αKey words
Hepatoma carcinoma cells/ Hypoxia/ Cell cycle/ Apoptosis/ Adhesion/ Migration/HIF-1α分类
医药卫生引用本文复制引用
王燕,李志伟,江萍,危群,易晓佳,陆晓青..低氧诱导对人肝癌SMMC7721细胞生物学行为的影响[J].昆明医学院学报,2011,32(5):40-46,7.基金项目
云南省应用基础研究基金资助项目(2005C0040Q) （2005C0040Q）
